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Comparing the disk-diffusion and agar dilution tests for Neisseria gonorrhoeae antimicrobial susceptibility testing

Overview of attention for article published in Antimicrobial Resistance & Infection Control, November 2016
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  • Above-average Attention Score compared to outputs of the same age (52nd percentile)

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Title
Comparing the disk-diffusion and agar dilution tests for Neisseria gonorrhoeae antimicrobial susceptibility testing
Published in
Antimicrobial Resistance & Infection Control, November 2016
DOI 10.1186/s13756-016-0148-x
Pubmed ID
Authors

Hsi Liu, Thomas H. Taylor, Kevin Pettus, Steve Johnson, John R. Papp, David Trees

Abstract

We assessed the validity of testing for antimicrobial susceptibility of clinical and mutant Neisseria gonorrhoeae (GC) isolates by disk diffusion in comparison to agar dilution, and Etest® (bioMerieux, France), respectively, for three third generation extended spectrum cephalosporins (ESC): ceftriaxone (CRO), cefixime (CFX), and cefpodoxime (CPD). One hundred and five clinical isolates and ten laboratory-mutants were tested following Clinical Laboratory Standard Institute (CLSI) and manufacturer's standards for each of the three methods. The measured diameters by the disk diffusion method were tested for correlation with the MIC values by agar dilution. In addition, comparisons with the Etest® were made. Categorical results for concordance, based on standard CLSI cutoffs, between the disk diffusion and the other two methods, respectively, were tested using the Chi-square statistics. Reproducibility was tested for CFX across a 6-month interval by repeated disk tests. Across all 115 specimens, the disk diffusion tests produced good categorical agreements, exhibiting concordance of 93.1%, 92.1%, and 90.4% with agar dilution and 93.0%, 92.1%, and 90.4% with Etest®, for CRO, CFX, and CPD, respectively. Pearson correlations between disk-diffusion diameters and agar dilution MIC's were -0.59, -0.67, and -0.81 for CRO, CFX, and CPD, respectively. The correlations between disk diffusion and Etest® were -0.58, -0.73, and -0.49. Pearson correlation between the CFX disk readings over a 6-month interval was 91%. Disk diffusion tests remain to be a useful, reliable and fast screening method for qualitative antimicrobial susceptibility testing for ceftriaxone, cefixime, and cefpodoxime.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 112 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 112 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 19 17%
Researcher 12 11%
Student > Ph. D. Student 9 8%
Student > Master 8 7%
Student > Doctoral Student 5 4%
Other 16 14%
Unknown 43 38%
Readers by discipline Count As %
Medicine and Dentistry 12 11%
Agricultural and Biological Sciences 11 10%
Pharmacology, Toxicology and Pharmaceutical Science 9 8%
Immunology and Microbiology 9 8%
Biochemistry, Genetics and Molecular Biology 5 4%
Other 18 16%
Unknown 48 43%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2021.
All research outputs
#13,536,909
of 24,003,070 outputs
Outputs from Antimicrobial Resistance & Infection Control
#777
of 1,347 outputs
Outputs of similar age
#200,194
of 421,897 outputs
Outputs of similar age from Antimicrobial Resistance & Infection Control
#24
of 32 outputs
Altmetric has tracked 24,003,070 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,347 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.6. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,897 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.