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Targeted bisulfite sequencing of the dynamic DNA methylome

Overview of attention for article published in Epigenetics & Chromatin, December 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (76th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (55th percentile)

Mentioned by

twitter
13 tweeters

Citations

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17 Dimensions

Readers on

mendeley
74 Mendeley
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Title
Targeted bisulfite sequencing of the dynamic DNA methylome
Published in
Epigenetics & Chromatin, December 2016
DOI 10.1186/s13072-016-0105-1
Pubmed ID
Authors

Michael J. Ziller, Elena K. Stamenova, Hongcang Gu, Andreas Gnirke, Alexander Meissner

Abstract

The ability to measure DNA methylation precisely and efficiently continues to drive our understanding of this modification in development and disease. Whole genome bisulfite sequencing has the advantage of theoretically capturing all cytosines in the genome at single-nucleotide resolution, but it has a number of significant practical drawbacks that become amplified with increasing sample numbers. All other technologies capture only a fraction of the cytosines that show dynamic regulation across cell and tissue types. Here, we present a novel hybrid selection design focusing on loci with dynamic methylation that captures a large number of differentially methylated gene-regulatory elements. We benchmarked this assay against matched whole genome data and profiled 25 human tissue samples to explore its ability to detect differentially methylated regions. Our target capture design fills a major gap left by all other assays that exist to map DNA methylation. It maintains the ability to link cytosine methylation to genetic differences, the single-base resolution and the analysis of neighboring cytosines while notably reducing the cost per sample by focusing the sequencing effort on the most informative and relevant regions of the genome.

Twitter Demographics

The data shown below were collected from the profiles of 13 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 74 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 27 36%
Student > Ph. D. Student 14 19%
Student > Master 8 11%
Other 7 9%
Student > Doctoral Student 5 7%
Other 7 9%
Unknown 6 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 33 45%
Agricultural and Biological Sciences 19 26%
Medicine and Dentistry 4 5%
Computer Science 2 3%
Pharmacology, Toxicology and Pharmaceutical Science 1 1%
Other 3 4%
Unknown 12 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2017.
All research outputs
#3,660,526
of 18,960,910 outputs
Outputs from Epigenetics & Chromatin
#163
of 515 outputs
Outputs of similar age
#94,800
of 406,813 outputs
Outputs of similar age from Epigenetics & Chromatin
#26
of 58 outputs
Altmetric has tracked 18,960,910 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 515 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 406,813 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.