Title |
Survey of domestic cattle for anti-Leishmania antibodies and LeishmaniaDNA in a visceral leishmaniasis endemic area of Bangladesh
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Published in |
BMC Veterinary Research, June 2011
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DOI | 10.1186/1746-6148-7-27 |
Pubmed ID | |
Authors |
Mohammad Shafiul Alam, Debashis Ghosh, Md Gulam Musawwir Khan, Mohammad Faizul Islam, Dinesh Mondal, Makoto Itoh, Md Nurul Islam, Rashidul Haque |
Abstract |
Visceral leishmaniasis (VL), caused by an intracellular parasite Leishmania donovani in the Indian subcontinent, is considered to be anthroponotic. The role of domestic animals in its transmission is still unclear. Although cattle are the preferred blood host for Phlebotomus argentipes, the sandfly vector of VL in the Indian subcontinent, very little information is available for their role in the disease transmission. In this study, we examined domestic cattle for serological and molecular evidence of Leishmania infection in a VL-endemic area in Bangladesh. Blood samples from 138 domestic cattle were collected from houses with active or recently-treated VL and post-kala-azar dermal leishmaniasis patients. The presence of anti-leishmanial antibodies in serum was investigated using enzyme-linked immunosorbent assay (ELISA) and then with direct agglutination tests (DAT). Nested PCR (Ln PCR) was performed to amplify the ssu-rRNA gene using the DNA extracted from Buffy coat. Recently-developed molecular assay loop-mediated isothermal amplification (LAMP) was also performed for further sensitive detection of parasite DNA. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Argentina | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Sri Lanka | 1 | 1% |
Bangladesh | 1 | 1% |
Canada | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 91 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 24 | 25% |
Student > Master | 13 | 14% |
Student > Ph. D. Student | 12 | 13% |
Student > Bachelor | 8 | 8% |
Student > Doctoral Student | 6 | 6% |
Other | 19 | 20% |
Unknown | 13 | 14% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 32 | 34% |
Medicine and Dentistry | 16 | 17% |
Biochemistry, Genetics and Molecular Biology | 9 | 9% |
Immunology and Microbiology | 7 | 7% |
Veterinary Science and Veterinary Medicine | 4 | 4% |
Other | 9 | 9% |
Unknown | 18 | 19% |