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Analysis of tilianin and acacetin in Agastache rugosa by high-performance liquid chromatography with ionic liquids-ultrasound based extraction

Overview of attention for article published in BMC Chemistry, November 2016
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Title
Analysis of tilianin and acacetin in Agastache rugosa by high-performance liquid chromatography with ionic liquids-ultrasound based extraction
Published in
BMC Chemistry, November 2016
DOI 10.1186/s13065-016-0223-7
Pubmed ID
Authors

Jinfeng Wei, Pengran Cao, Jinmei Wang, Wenyi Kang

Abstract

Ionic liquid 1-butyl-3-methylimidazolium bromide-methanol-based ultrasonic-assisted extraction (ILUAE) was used to extract tilianin and acacetin from the aerial parts of Agastache rugose (A. rugose), and simultaneously determined by reversed phase high performance liquid chromatographic (RP-HPLC) method with ultraviolet detection (RP-HPLC-UV). An InertSustain RP-C18 column was used with the mobile phase consisting of methanol and 0.2% acetic acid as gradient elution at the detection wavelength of 332 nm. The flow rate was 0.8 mL/min, and the column temperature was 30 °C. Under the optimized conditions, tilianin and acacetin displayed good linearity in the ranges of 0.0595-4.76 and 0.0585-4.68 μg/mL, respectively, with the average recoveries being 96.93 and 97.88%, respectively. The method of ILUAE was compared with the traditional methods, it exhibited higher efficiency, higher reproducibility and environmental friendly in analyzing the active compounds in traditional Chinese medicines (TCMs).

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The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 22%
Professor > Associate Professor 2 11%
Student > Ph. D. Student 1 6%
Other 1 6%
Researcher 1 6%
Other 1 6%
Unknown 8 44%
Readers by discipline Count As %
Chemistry 3 17%
Pharmacology, Toxicology and Pharmaceutical Science 2 11%
Biochemistry, Genetics and Molecular Biology 2 11%
Environmental Science 1 6%
Energy 1 6%
Other 1 6%
Unknown 8 44%