Title |
A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs
|
---|---|
Published in |
BMC Biotechnology, May 2013
|
DOI | 10.1186/1472-6750-13-41 |
Pubmed ID | |
Authors |
Ashley R Long, Catherine C O’Brien, Ketan Malhotra, Christine T Schwall, Arlene D Albert, Anthony Watts, Nathan N Alder |
Abstract |
The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate membrane proteins. Exposure to detergents, however, can destabilize many membrane proteins and result in a loss of function. Amphipathic copolymers have recently been used to stabilize membrane proteins and complexes following suitable detergent extraction. However, the ability of these copolymers to extract proteins directly from native lipid bilayers for subsequent reconstitution and characterization has not been explored. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 3 | 1% |
United States | 2 | <1% |
Denmark | 2 | <1% |
Germany | 2 | <1% |
Unknown | 222 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 64 | 28% |
Researcher | 50 | 22% |
Student > Master | 28 | 12% |
Student > Bachelor | 18 | 8% |
Student > Doctoral Student | 11 | 5% |
Other | 29 | 13% |
Unknown | 31 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 74 | 32% |
Biochemistry, Genetics and Molecular Biology | 67 | 29% |
Chemistry | 36 | 16% |
Engineering | 6 | 3% |
Physics and Astronomy | 4 | 2% |
Other | 12 | 5% |
Unknown | 32 | 14% |