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A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs

Overview of attention for article published in BMC Biotechnology, January 2013
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (55th percentile)
  • Good Attention Score compared to outputs of the same age and source (66th percentile)

Mentioned by

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1 tweeter
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1 research highlight platform

Citations

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96 Dimensions

Readers on

mendeley
203 Mendeley
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Title
A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs
Published in
BMC Biotechnology, January 2013
DOI 10.1186/1472-6750-13-41
Pubmed ID
Authors

Ashley R Long, Catherine C O’Brien, Ketan Malhotra, Christine T Schwall, Arlene D Albert, Anthony Watts, Nathan N Alder

Abstract

The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate membrane proteins. Exposure to detergents, however, can destabilize many membrane proteins and result in a loss of function. Amphipathic copolymers have recently been used to stabilize membrane proteins and complexes following suitable detergent extraction. However, the ability of these copolymers to extract proteins directly from native lipid bilayers for subsequent reconstitution and characterization has not been explored.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 203 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 1%
Germany 2 <1%
United States 2 <1%
Denmark 2 <1%
Poland 1 <1%
Unknown 193 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 57 28%
Researcher 46 23%
Student > Master 25 12%
Student > Bachelor 18 9%
Student > Doctoral Student 10 5%
Other 27 13%
Unknown 20 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 74 36%
Biochemistry, Genetics and Molecular Biology 58 29%
Chemistry 31 15%
Engineering 6 3%
Physics and Astronomy 4 2%
Other 9 4%
Unknown 21 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2013.
All research outputs
#4,281,933
of 8,891,989 outputs
Outputs from BMC Biotechnology
#245
of 504 outputs
Outputs of similar age
#51,454
of 122,114 outputs
Outputs of similar age from BMC Biotechnology
#4
of 12 outputs
Altmetric has tracked 8,891,989 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 504 research outputs from this source. They receive a mean Attention Score of 4.5. This one is in the 46th percentile – i.e., 46% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 122,114 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.