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A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs

Overview of attention for article published in BMC Biotechnology, May 2013
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Mentioned by

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1 tweeter
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1 research highlight platform

Citations

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107 Dimensions

Readers on

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223 Mendeley
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Title
A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs
Published in
BMC Biotechnology, May 2013
DOI 10.1186/1472-6750-13-41
Pubmed ID
Authors

Ashley R Long, Catherine C O’Brien, Ketan Malhotra, Christine T Schwall, Arlene D Albert, Anthony Watts, Nathan N Alder

Abstract

The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate membrane proteins. Exposure to detergents, however, can destabilize many membrane proteins and result in a loss of function. Amphipathic copolymers have recently been used to stabilize membrane proteins and complexes following suitable detergent extraction. However, the ability of these copolymers to extract proteins directly from native lipid bilayers for subsequent reconstitution and characterization has not been explored.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 223 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 1%
United States 2 <1%
Denmark 2 <1%
Germany 2 <1%
Unknown 214 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 62 28%
Researcher 49 22%
Student > Master 28 13%
Student > Bachelor 19 9%
Student > Doctoral Student 11 5%
Other 27 12%
Unknown 27 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 74 33%
Biochemistry, Genetics and Molecular Biology 65 29%
Chemistry 35 16%
Engineering 6 3%
Physics and Astronomy 4 2%
Other 11 5%
Unknown 28 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2013.
All research outputs
#11,164,396
of 17,829,175 outputs
Outputs from BMC Biotechnology
#594
of 874 outputs
Outputs of similar age
#91,260
of 164,452 outputs
Outputs of similar age from BMC Biotechnology
#1
of 1 outputs
Altmetric has tracked 17,829,175 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 874 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 164,452 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them