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A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs

Overview of attention for article published in BMC Biotechnology, May 2013
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1 X user
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Citations

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231 Mendeley
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Title
A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs
Published in
BMC Biotechnology, May 2013
DOI 10.1186/1472-6750-13-41
Pubmed ID
Authors

Ashley R Long, Catherine C O’Brien, Ketan Malhotra, Christine T Schwall, Arlene D Albert, Anthony Watts, Nathan N Alder

Abstract

The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate membrane proteins. Exposure to detergents, however, can destabilize many membrane proteins and result in a loss of function. Amphipathic copolymers have recently been used to stabilize membrane proteins and complexes following suitable detergent extraction. However, the ability of these copolymers to extract proteins directly from native lipid bilayers for subsequent reconstitution and characterization has not been explored.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 231 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 3 1%
United States 2 <1%
Denmark 2 <1%
Germany 2 <1%
Unknown 222 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 64 28%
Researcher 50 22%
Student > Master 28 12%
Student > Bachelor 18 8%
Student > Doctoral Student 11 5%
Other 29 13%
Unknown 31 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 74 32%
Biochemistry, Genetics and Molecular Biology 67 29%
Chemistry 36 16%
Engineering 6 3%
Physics and Astronomy 4 2%
Other 12 5%
Unknown 32 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2013.
All research outputs
#15,557,505
of 23,881,329 outputs
Outputs from BMC Biotechnology
#664
of 937 outputs
Outputs of similar age
#117,587
of 195,645 outputs
Outputs of similar age from BMC Biotechnology
#10
of 17 outputs
Altmetric has tracked 23,881,329 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 937 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.9. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 195,645 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.