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Limb patterning genes and heterochronic development of the emu wing bud

Overview of attention for article published in EvoDevo, December 2016
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Limb patterning genes and heterochronic development of the emu wing bud
Published in
EvoDevo, December 2016
DOI 10.1186/s13227-016-0063-5
Pubmed ID

Craig A. Smith, Peter G. Farlie, Nadia M. Davidson, Kelly N. Roeszler, Claire Hirst, Alicia Oshlack, David M. Lambert


The forelimb of the flightless emu is a vestigial structure, with greatly reduced wing elements and digit loss. To explore the molecular and cellular mechanisms associated with the evolution of vestigial wings and loss of flight in the emu, key limb patterning genes were examined in developing embryos. Limb development was compared in emu versus chicken embryos. Immunostaining for cell proliferation markers was used to analyze growth of the emu forelimb and hindlimb buds. Expression patterns of limb patterning genes were studied, using whole-mount in situ hybridization (for mRNA localization) and RNA-seq (for mRNA expression levels). The forelimb of the emu embryo showed heterochronic development compared to that in the chicken, with the forelimb bud being retarded in its development. Early outgrowth of the emu forelimb bud is characterized by a lower level of cell proliferation compared the hindlimb bud, as assessed by PH3 immunostaining. In contrast, there were no obvious differences in apoptosis in forelimb versus hindlimb buds (cleaved caspase 3 staining). Most key patterning genes were expressed in emu forelimb buds similarly to that observed in the chicken, but with smaller expression domains. However, expression of Sonic Hedgehog (Shh) mRNA, which is central to anterior-posterior axis development, was delayed in the emu forelimb bud relative to other patterning genes. Regulators of Shh expression, Gli3 and HoxD13, also showed altered expression levels in the emu forelimb bud. These data reveal heterochronic but otherwise normal expression of most patterning genes in the emu vestigial forelimb. Delayed Shh expression may be related to the small and vestigial structure of the emu forelimb bud. However, the genetic mechanism driving retarded emu wing development is likely to rest within the forelimb field of the lateral plate mesoderm, predating the expression of patterning genes.

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Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
Spain 1 2%
Unknown 39 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 22%
Researcher 6 15%
Student > Bachelor 6 15%
Student > Doctoral Student 2 5%
Other 2 5%
Other 8 20%
Unknown 8 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 34%
Biochemistry, Genetics and Molecular Biology 12 29%
Unspecified 1 2%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Earth and Planetary Sciences 1 2%
Other 2 5%
Unknown 10 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 December 2016.
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