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Characterization of a novel swollenin from Penicillium oxalicum in facilitating enzymatic saccharification of cellulose

Overview of attention for article published in BMC Biotechnology, May 2013
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  • Good Attention Score compared to outputs of the same age (67th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (62nd percentile)

Mentioned by

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1 X user
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1 Wikipedia page

Citations

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55 Dimensions

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96 Mendeley
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Title
Characterization of a novel swollenin from Penicillium oxalicum in facilitating enzymatic saccharification of cellulose
Published in
BMC Biotechnology, May 2013
DOI 10.1186/1472-6750-13-42
Pubmed ID
Authors

Kang Kang, Shaowen Wang, Guohong Lai, Gang Liu, Miao Xing

Abstract

BACKGROUND: Plant expansins and fungal swollenin that can disrupt crystalline cellulose have great potential for applications in conversion of biomass. Recent studies have been mainly focused on Trichoderma reesei swollenin that show relatively low activity in the promotion of cellulosic hydrolysis. Our aim was to isolate a novel swollenin with greater disruptive activity, to establish an efficient way of producing recombinant swollenin, and to optimize the procedure using swollenin in facilitation of cellulosic hydrolysis. RESULTS: A novel gene encoding a swollenin-like protein, POSWOI, was isolated from the filamentous fungus Penicillium oxalicum by Thermal Asymmetric Interlaced PCR (TAIL-PCR). It consisted of a family 1 carbohydrate-binding module (CBM1) followed by a linker connected to a family 45 endoglucanase-like domain. Using the cellobiohydrolase I promoter, recombinant POSWOI was efficiently produced in T. reesei with a yield of 105 mg/mL, and showed significant disruptive activity on crystalline cellulose. Simultaneous reaction with both POSWOI and cellulases enhanced the hydrolysis of crystalline cellulose Avicel by approximately 50%. Using a POSWOI-pretreatment procedure, cellulases can produce nearly twice as many reducing sugars as without pretreatment. The mechanism by which POSWOI facilitates the saccharification of cellulose was also studied using a cellulase binding assay. CONCLUSION: We present a novel fungal swollenin with considerable disruptive activity on crystalline cellulose, and develop a better procedure for using swollenin in facilitating cellulosic hydrolysis. We thus provide a new approach for the effective bioconversion of cellulosic biomass.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 96 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 1%
Italy 1 1%
Finland 1 1%
Denmark 1 1%
United States 1 1%
Unknown 91 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 28 29%
Researcher 16 17%
Student > Master 15 16%
Student > Bachelor 6 6%
Student > Postgraduate 5 5%
Other 15 16%
Unknown 11 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 43 45%
Biochemistry, Genetics and Molecular Biology 24 25%
Engineering 4 4%
Environmental Science 2 2%
Chemical Engineering 1 1%
Other 4 4%
Unknown 18 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 October 2023.
All research outputs
#7,653,291
of 24,584,609 outputs
Outputs from BMC Biotechnology
#412
of 967 outputs
Outputs of similar age
#61,696
of 199,674 outputs
Outputs of similar age from BMC Biotechnology
#6
of 16 outputs
Altmetric has tracked 24,584,609 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 967 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 199,674 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.