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The Coptotermes gestroi aldo–keto reductase: a multipurpose enzyme for biorefinery applications

Overview of attention for article published in Biotechnology for Biofuels and Bioproducts, January 2017
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Title
The Coptotermes gestroi aldo–keto reductase: a multipurpose enzyme for biorefinery applications
Published in
Biotechnology for Biofuels and Bioproducts, January 2017
DOI 10.1186/s13068-016-0688-6
Pubmed ID
Authors

Robson Tramontina, João Paulo L. Franco Cairo, Marcelo V. Liberato, Fernanda Mandelli, Amanda Sousa, Samantha Santos, Sarita Cândida Rabelo, Bruna Campos, Jaciane Ienczak, Roberto Ruller, André R. L. Damásio, Fabio Marcio Squina

Abstract

In nature, termites can be considered as a model biological system for biofuel research based on their remarkable efficiency for lignocellulosic biomass conversion. Redox enzymes are of interest in second-generation ethanol production because they promote synergic enzymatic activity with classical hydrolases for lignocellulose saccharification and inactivate fermentation inhibitory compounds produced after lignocellulose pretreatment steps. In the present study, the biochemical and structural characteristics of the Coptotermes gestroi aldo-keto reductase (CgAKR-1) were comprehensively investigated. CgAKR-1 displayed major structural differences compared with others AKRs, including the differences in the amino acid composition of the substrate-binding site, providing basis for classification as a founding member of a new AKR subfamily (family AKR1 I). Immunolocalization assays with anti-CgAKR-1 antibodies resulted in strong fluorescence in the salivary gland, proventriculus, and foregut. CgAKR-1 supplementation caused a 32% reduction in phenolic aldehydes, such as furfural, which act as fermentation inhibitors of hemicellulosic hydrolysates, and improved ethanol fermentation by the xylose-fermenting yeast Scheffersomyces stipitis by 45%. We observed synergistic enzymatic interactions between CgAKR-1 and commercial cellulosic cocktail for sugarcane bagasse saccharification, with a maximum synergism degree of 2.17 for sugar release. Our data indicated that additive enzymatic activity could be mediated by reactive oxygen species because CgAKR-1 could produce hydrogen peroxide. In summary, we identified the founding member of an AKRI subfamily with a potential role in the termite digestome. CgAKR-1 was found to be a multipurpose enzyme with potential biotechnological applications. The present work provided a basis for the development and application of integrative and multipurpose enzymes in the bioethanol production chain.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 1%
Unknown 78 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 14%
Researcher 11 14%
Student > Master 10 13%
Student > Bachelor 8 10%
Student > Doctoral Student 5 6%
Other 18 23%
Unknown 16 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 25%
Biochemistry, Genetics and Molecular Biology 17 22%
Chemistry 5 6%
Chemical Engineering 3 4%
Environmental Science 3 4%
Other 13 16%
Unknown 18 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 January 2017.
All research outputs
#17,285,036
of 25,373,627 outputs
Outputs from Biotechnology for Biofuels and Bioproducts
#997
of 1,578 outputs
Outputs of similar age
#268,087
of 422,324 outputs
Outputs of similar age from Biotechnology for Biofuels and Bioproducts
#30
of 51 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,578 research outputs from this source. They receive a mean Attention Score of 4.9. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 422,324 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 51 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.