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Chemical composition, antimicrobial properties and toxicity evaluation of the essential oil of Cupressus lusitanica Mill. leaves from Cameroon

Overview of attention for article published in BMC Complementary Medicine and Therapies, June 2013
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Title
Chemical composition, antimicrobial properties and toxicity evaluation of the essential oil of Cupressus lusitanica Mill. leaves from Cameroon
Published in
BMC Complementary Medicine and Therapies, June 2013
DOI 10.1186/1472-6882-13-130
Pubmed ID
Authors

Gerald Ngo Teke, Kemadjou Nana Elisée, Kuiate Jules Roger

Abstract

BACKGROUND: The leaves of Cupressus lusitanica Mill. are used in the western highlands of Cameroon for their medicinal property. METHODS: The leaves of this species were collected in the West Region of Cameroon in August 2010 and subjected to hydrodistillation to obtain the essential oil. The oil was fractionated using adsorption column chromatography. The chemical composition of this oil and its fractions was analysed by gas chromatography--mass spectrometry (GC-MS). The essential oil and fractions were tested for antimicrobial activity against eight bacterial species and six species of Candida by the agar diffusion method. Macrodilution method was used to determine the minimum inhibition concentrations (MICs) and minimum bactericidal and/or fungicidal concentrations (MBCs and MFCs). The toxicity profile of the oil was studied using Swiss mice and Wistar albino rats. RESULTS: Forty-nine compounds were identified in the essential oil. The main components were germacrene D (18.5%), epi-zonarene (8.2%), cis-calamenene (8.2%), terpinen-4-ol (6.3%), linalool (6.0%) and umbellulone (6.0%). Enterococcus faecalis, Proteus mirabilis and Candida albicans were most susceptible to the oil (MICs of 1.25 and 0.16% for bacteria and fungi respectively). The estimated oral LD50 was 6.33 g/kg. There was an increase in sera ALT and AST activities while the blood cells and protein levels decreased in treated animals. CONCLUSION: The results obtained from this study support the ethnomedicinal use of C. lusitanica leaf oil in the treatment of whooping cough and skin infections though it should be used with care. This plant oil could be useful in the standardisation of phytomedicine.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Poland 1 1%
Unknown 71 99%

Demographic breakdown

Readers by professional status Count As %
Student > Master 15 21%
Researcher 9 13%
Student > Bachelor 6 8%
Student > Doctoral Student 5 7%
Other 4 6%
Other 13 18%
Unknown 20 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 19%
Pharmacology, Toxicology and Pharmaceutical Science 9 13%
Biochemistry, Genetics and Molecular Biology 7 10%
Chemistry 6 8%
Medicine and Dentistry 6 8%
Other 5 7%
Unknown 25 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2014.
All research outputs
#15,117,945
of 23,999,200 outputs
Outputs from BMC Complementary Medicine and Therapies
#1,728
of 3,769 outputs
Outputs of similar age
#114,442
of 200,070 outputs
Outputs of similar age from BMC Complementary Medicine and Therapies
#52
of 97 outputs
Altmetric has tracked 23,999,200 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,769 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.1. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 200,070 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 97 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.