Title |
Transcriptomic signatures differentiate survival from fatal outcomes in humans infected with Ebola virus
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Published in |
Genome Biology, January 2017
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DOI | 10.1186/s13059-016-1137-3 |
Pubmed ID | |
Authors |
Xuan Liu, Emily Speranza, César Muñoz-Fontela, Sam Haldenby, Natasha Y. Rickett, Isabel Garcia-Dorival, Yongxiang Fang, Yper Hall, Elsa-Gayle Zekeng, Anja Lüdtke, Dong Xia, Romy Kerber, Ralf Krumkamp, Sophie Duraffour, Daouda Sissoko, John Kenny, Nichola Rockliffe, E. Diane Williamson, Thomas R. Laws, Magassouba N’Faly, David A. Matthews, Stephan Günther, Andrew R. Cossins, Armand Sprecher, John H. Connor, Miles W. Carroll, Julian A. Hiscox |
Abstract |
In 2014, Western Africa experienced an unanticipated explosion of Ebola virus infections. What distinguishes fatal from non-fatal outcomes remains largely unknown, yet is key to optimising personalised treatment strategies. We used transcriptome data for peripheral blood taken from infected and convalescent recovering patients to identify early stage host factors that are associated with acute illness and those that differentiate patient survival from fatality. The data demonstrate that individuals who succumbed to the disease show stronger upregulation of interferon signalling and acute phase responses compared to survivors during the acute phase of infection. Particularly notable is the strong upregulation of albumin and fibrinogen genes, which suggest significant liver pathology. Cell subtype prediction using messenger RNA expression patterns indicated that NK-cell populations increase in patients who survive infection. By selecting genes whose expression properties discriminated between fatal cases and survivors, we identify a small panel of responding genes that act as strong predictors of patient outcome, independent of viral load. Transcriptomic analysis of the host response to pathogen infection using blood samples taken during an outbreak situation can provide multiple levels of information on both disease state and mechanisms of pathogenesis. Host biomarkers were identified that provide high predictive value under conditions where other predictors, such as viral load, are poor prognostic indicators. The data suggested that rapid analysis of the host response to infection in an outbreak situation can provide valuable information to guide an understanding of disease outcome and mechanisms of disease. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 8 | 22% |
United States | 7 | 19% |
Canada | 2 | 6% |
Switzerland | 1 | 3% |
France | 1 | 3% |
Spain | 1 | 3% |
Sweden | 1 | 3% |
Curaçao | 1 | 3% |
Unknown | 14 | 39% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 22 | 61% |
Scientists | 9 | 25% |
Science communicators (journalists, bloggers, editors) | 3 | 8% |
Practitioners (doctors, other healthcare professionals) | 2 | 6% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 2% |
United Kingdom | 2 | 1% |
Unknown | 153 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 35 | 22% |
Student > Ph. D. Student | 22 | 14% |
Student > Bachelor | 17 | 11% |
Student > Master | 16 | 10% |
Student > Doctoral Student | 9 | 6% |
Other | 29 | 18% |
Unknown | 30 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 28 | 18% |
Immunology and Microbiology | 24 | 15% |
Agricultural and Biological Sciences | 23 | 15% |
Medicine and Dentistry | 18 | 11% |
Computer Science | 4 | 3% |
Other | 22 | 14% |
Unknown | 39 | 25% |