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Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli

Overview of attention for article published in BMC Biotechnology, June 2013
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Mentioned by

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1 tweeter

Citations

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17 Dimensions

Readers on

mendeley
14 Mendeley
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Title
Strategy for successful expression of the Pseudomonas putida nitrile hydratase activator P14K in Escherichia coli
Published in
BMC Biotechnology, June 2013
DOI 10.1186/1472-6750-13-48
Pubmed ID
Authors

Yi Liu, Wenjing Cui, Yueqin Fang, Yuechun Yu, Youtian Cui, Yuanyuan Xia, Michihiko Kobayashi, Zhemin Zhou

Abstract

Activators of Nitrile hydratase (NHase) are essential for functional NHase biosynthesis. However, the activator P14K in P. putida is difficult to heterogeneously express, which retards the clarification of the mechanism of P14K involved in the maturation of NHase. Although a strep tag containing P14K (strep-P14K) was over-expressed, its low expression level and low stability affect the further analysis.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 7%
Unknown 13 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 21%
Student > Doctoral Student 2 14%
Student > Ph. D. Student 2 14%
Lecturer 1 7%
Professor 1 7%
Other 4 29%
Unknown 1 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 36%
Biochemistry, Genetics and Molecular Biology 5 36%
Environmental Science 1 7%
Chemistry 1 7%
Engineering 1 7%
Other 0 0%
Unknown 1 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 June 2013.
All research outputs
#2,017,626
of 3,628,172 outputs
Outputs from BMC Biotechnology
#163
of 298 outputs
Outputs of similar age
#45,033
of 85,434 outputs
Outputs of similar age from BMC Biotechnology
#4
of 9 outputs
Altmetric has tracked 3,628,172 research outputs across all sources so far. This one is in the 25th percentile – i.e., 25% of other outputs scored the same or lower than it.
So far Altmetric has tracked 298 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 85,434 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.