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High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI

Overview of attention for article published in BMC Genomic Data, June 2013
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#46 of 1,204)
  • High Attention Score compared to outputs of the same age (92nd percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

Mentioned by

blogs
1 blog
twitter
4 X users
patent
4 patents

Citations

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20 Dimensions

Readers on

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42 Mendeley
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Title
High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI
Published in
BMC Genomic Data, June 2013
DOI 10.1186/1471-2156-14-56
Pubmed ID
Authors

Xiaojun Huang, Hanlin Lu, Jun-Wen Wang, Liqin Xu, Siyang Liu, Jihua Sun, Fei Gao

Abstract

As a well-known epigenomic modification, DNA methylation is found to be common in plants and plays an important role in many biological processes. Relying on the unique feature of methylation-dependent digestion, the family of methylation-requiring restriction-like endonuclease, such as MspJI and its homologs, was suggested for a potential usage in methylation detection. In this study, we combine MspJI digestion and electrophoretic band selection with next generation high-throughput sequencing technology to detect 5-methylcytosines in Arabidopsis genome. By developing a bioinformatics workflow to attribute the CNNR sites recognized by MspJI to the reference genome, we fulfilled the systematic assessment of this method. According to the assessment, here we provide the method for generating a detailed map of plant methylome that could be feasible, reliable and economical in methylation investigation.

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Unknown 41 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 26%
Student > Ph. D. Student 11 26%
Student > Bachelor 5 12%
Student > Master 5 12%
Student > Doctoral Student 3 7%
Other 6 14%
Unknown 1 2%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 52%
Biochemistry, Genetics and Molecular Biology 9 21%
Engineering 4 10%
Computer Science 2 5%
Nursing and Health Professions 1 2%
Other 3 7%
Unknown 1 2%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 November 2019.
All research outputs
#1,953,957
of 25,405,598 outputs
Outputs from BMC Genomic Data
#46
of 1,204 outputs
Outputs of similar age
#16,295
of 209,390 outputs
Outputs of similar age from BMC Genomic Data
#2
of 20 outputs
Altmetric has tracked 25,405,598 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,204 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 209,390 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.