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High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI

Overview of attention for article published in BMC Genetics, January 2013
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#34 of 1,033)
  • High Attention Score compared to outputs of the same age (92nd percentile)

Mentioned by

blogs
1 blog
twitter
5 tweeters
patent
4 patents

Citations

dimensions_citation
13 Dimensions

Readers on

mendeley
38 Mendeley
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Title
High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI
Published in
BMC Genetics, January 2013
DOI 10.1186/1471-2156-14-56
Pubmed ID
Authors

Xiaojun Huang, Hanlin Lu, Jun-Wen Wang, Liqin Xu, Siyang Liu, Jihua Sun, Fei Gao

Abstract

As a well-known epigenomic modification, DNA methylation is found to be common in plants and plays an important role in many biological processes. Relying on the unique feature of methylation-dependent digestion, the family of methylation-requiring restriction-like endonuclease, such as MspJI and its homologs, was suggested for a potential usage in methylation detection. In this study, we combine MspJI digestion and electrophoretic band selection with next generation high-throughput sequencing technology to detect 5-methylcytosines in Arabidopsis genome. By developing a bioinformatics workflow to attribute the CNNR sites recognized by MspJI to the reference genome, we fulfilled the systematic assessment of this method. According to the assessment, here we provide the method for generating a detailed map of plant methylome that could be feasible, reliable and economical in methylation investigation.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Unknown 37 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 29%
Researcher 10 26%
Student > Master 5 13%
Student > Bachelor 5 13%
Student > Doctoral Student 3 8%
Other 4 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 58%
Biochemistry, Genetics and Molecular Biology 8 21%
Engineering 4 11%
Computer Science 2 5%
Nursing and Health Professions 1 3%
Other 1 3%

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 November 2019.
All research outputs
#1,212,801
of 17,365,229 outputs
Outputs from BMC Genetics
#34
of 1,033 outputs
Outputs of similar age
#12,213
of 163,669 outputs
Outputs of similar age from BMC Genetics
#1
of 1 outputs
Altmetric has tracked 17,365,229 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,033 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 163,669 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them