Title |
Simple extraction methods that prevent the artifactual conversion of chlorophyll to chlorophyllide during pigment isolation from leaf samples
|
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Published in |
Plant Methods, June 2013
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DOI | 10.1186/1746-4811-9-19 |
Pubmed ID | |
Authors |
Xueyun Hu, Ayumi Tanaka, Ryouichi Tanaka |
Abstract |
When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely used for this purpose because it provides an accurate determination of a variety of photosynthetic pigments simultaneously. This technique has a drawback compared with conventional spectroscopic techniques, however, in that it is more prone to structural modification of pigments during extraction, thus potentially generating erroneous results. During pigment extraction procedures with acetone or alcohol, the phytol side chain of chlorophyll is sometimes removed, forming chlorophyllide, which affects chlorophyll measurement using HPLC. |
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Spain | 1 | 17% |
Unknown | 3 | 50% |
Demographic breakdown
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Science communicators (journalists, bloggers, editors) | 1 | 17% |
Scientists | 1 | 17% |
Mendeley readers
Geographical breakdown
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Switzerland | 1 | <1% |
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Sri Lanka | 1 | <1% |
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Researcher | 31 | 10% |
Student > Doctoral Student | 10 | 3% |
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Unknown | 66 | 21% |
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Engineering | 7 | 2% |
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