Title |
A fluorometric assay for trehalose in the picomole range
|
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Published in |
Plant Methods, June 2013
|
DOI | 10.1186/1746-4811-9-21 |
Pubmed ID | |
Authors |
Petronia Carillo, Regina Feil, Yves Gibon, Namiko Satoh-Nagasawa, David Jackson, Oliver E Bläsing, Mark Stitt, John Edward Lunn |
Abstract |
Trehalose is a non-reducing disaccharide that is used as an osmolyte, transport sugar, carbon reserve and stress protectant in a wide range of organisms. In plants, trehalose 6-phosphate (Tre6P), the intermediate of trehalose biosynthesis, is thought to be a signal of sucrose status. Trehalose itself may play a role in pathogenic and symbiotic plant-microbe interactions, in responses to abiotic stress and in developmental signalling, but its precise functions are unknown. A major obstacle to investigating its function is the technical difficulty of measuring the very low levels of trehalose usually found in plant tissues, as most of the established trehalose assays lack sufficient specificity and/or sensitivity. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 4 | 3% |
Hungary | 1 | <1% |
Germany | 1 | <1% |
United Kingdom | 1 | <1% |
India | 1 | <1% |
Belgium | 1 | <1% |
New Zealand | 1 | <1% |
Unknown | 112 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 35 | 29% |
Researcher | 26 | 21% |
Student > Master | 13 | 11% |
Professor > Associate Professor | 7 | 6% |
Student > Bachelor | 7 | 6% |
Other | 20 | 16% |
Unknown | 14 | 11% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 73 | 60% |
Biochemistry, Genetics and Molecular Biology | 18 | 15% |
Chemistry | 5 | 4% |
Environmental Science | 3 | 2% |
Immunology and Microbiology | 2 | 2% |
Other | 5 | 4% |
Unknown | 16 | 13% |