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Application of hairless mouse strain to bioluminescence imaging of Arc expression in mouse brain

Overview of attention for article published in BMC Neuroscience, January 2017
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Title
Application of hairless mouse strain to bioluminescence imaging of Arc expression in mouse brain
Published in
BMC Neuroscience, January 2017
DOI 10.1186/s12868-017-0335-6
Pubmed ID
Authors

Hironori Izumi, Tetsuya Ishimoto, Hiroshi Yamamoto, Hisashi Mori

Abstract

Bioluminescence imaging (BLI) is a powerful technique for monitoring the temporal and spatial dynamics of gene expression in the mouse brain. However, the black fur, skin pigmentation and hair regrowth after depilation of mouse interfere with BLI during developmental and daily examination. The aim of this study was to extend the application of Arc-Luc transgenic (Tg) mice to the BLI of neuronal activity in the mouse brain by introducing the hairless (HL) gene and to examine Arc-Luc expression at various developmental stages without interference from black fur, skin pigmentation, and hair regrowth. The Arc-Luc Tg HL mice were established by crossing the Tg C57BL/6 mouse strain with the HL mouse strain. Under physiological and pathological conditions, BLI was performed to detect the signal intensity changes at various developmental stages and at an interval of <7 days. The established Arc-Luc Tg HL mice exhibited clear and stable photon signals from the brain without interference during development. After surgical monocular deprivation during visual-critical period, large signal intensity changes in bioluminescence were observed in the mouse visual cortex. Exposure of mice to a novel object changed the photon distribution in the caudal and rostral cerebral areas. The temporal pattern of kainic-acid-induced Arc-Luc expression showed biphasic changes in signal intensity over 24 h. This study showed the advantages of using the mutant HL gene in BLI of Arc expression in the mouse brain at various developmental stages. Thus, the use of the Arc-Luc Tg HL mice enabled the tracking of neuronal-activity-dependent processes over a wide range from a focal area to the entire brain area with various time windows.

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Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Librarian 2 29%
Researcher 2 29%
Student > Ph. D. Student 1 14%
Student > Doctoral Student 1 14%
Unknown 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 14%
Business, Management and Accounting 1 14%
Agricultural and Biological Sciences 1 14%
Immunology and Microbiology 1 14%
Medicine and Dentistry 1 14%
Other 0 0%
Unknown 2 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 January 2017.
All research outputs
#9,169,230
of 10,444,782 outputs
Outputs from BMC Neuroscience
#787
of 936 outputs
Outputs of similar age
#262,474
of 318,576 outputs
Outputs of similar age from BMC Neuroscience
#14
of 32 outputs
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