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Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites

Overview of attention for article published in Parasites & Vectors, January 2017
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Title
Ultra-sensitive chemiluminescence imaging DNA hybridization method in the detection of mosquito-borne viruses and parasites
Published in
Parasites & Vectors, January 2017
DOI 10.1186/s13071-017-1975-1
Pubmed ID
Authors

Yingjie Zhang, Qiqi Liu, Biao Zhou, Xiaobo Wang, Suhong Chen, Shengqi Wang

Abstract

Mosquito-borne viruses (MBVs) and parasites (MBPs) are transmitted through hematophagous arthropods-mosquitoes to homoiothermous vertebrates. This study aims at developing a detection method to monitor the spread of mosquito-borne diseases to new areas and diagnose the infections caused by MBVs and MBPs. In this assay, an ultra-sensitive chemiluminescence (CL) detection method was developed and used to simultaneously detect 19 common MBVs and MBPs. In vitro transcript RNA, virus-like particles (VLPs), and plasmids were established as positive or limit of detection (LOD) reference materials. MBVs and MBPs could be genotyped with high sensitivity and specificity. The cut-off values of probes were calculated. The absolute LODs of this strategy to detect serially diluted in vitro transcribed RNAs of MBVs and serially diluted plasmids of MBPs were 10(2)-10(3)copies/μl and 10(1)-10(2)copies/μl, respectively. Further, the LOD of detecting a strain of pre-quantified JEV was 10(1.8)-10(0.8)PFU/ml, fitted well in a linear regression model (coefficient of determination = 0.9678). Ultra-sensitive CL imaging DNA hybridization was developed and could simultaneously detect various MBVs and MBPs. The method described here has the potential to provide considerable labor savings due to its ability to screen for 19 mosquito-borne pathogens simultaneously.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 27%
Student > Ph. D. Student 5 19%
Researcher 3 12%
Student > Master 2 8%
Unknown 9 35%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 23%
Medicine and Dentistry 4 15%
Biochemistry, Genetics and Molecular Biology 2 8%
Mathematics 1 4%
Veterinary Science and Veterinary Medicine 1 4%
Other 2 8%
Unknown 10 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 January 2017.
All research outputs
#18,525,776
of 22,947,506 outputs
Outputs from Parasites & Vectors
#4,248
of 5,483 outputs
Outputs of similar age
#309,801
of 419,016 outputs
Outputs of similar age from Parasites & Vectors
#91
of 122 outputs
Altmetric has tracked 22,947,506 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,483 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 419,016 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 122 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.