Title |
Methylome reorganization during in vitro dedifferentiation and regeneration of Populus trichocarpa
|
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Published in |
BMC Plant Biology, June 2013
|
DOI | 10.1186/1471-2229-13-92 |
Pubmed ID | |
Authors |
Kelly Vining, Kyle R Pomraning, Larry J Wilhelm, Cathleen Ma, Matteo Pellegrini, Yanming Di, Todd C Mockler, Michael Freitag, Steven H Strauss |
Abstract |
Cytosine DNA methylation (5mC) is an epigenetic modification that is important to genome stability and regulation of gene expression. Perturbations of 5mC have been implicated as a cause of phenotypic variation among plants regenerated through in vitro culture systems. However, the pattern of change in 5mC and its functional role with respect to gene expression, are poorly understood at the genome scale. A fuller understanding of how 5mC changes during in vitro manipulation may aid the development of methods for reducing or amplifying the mutagenic and epigenetic effects of in vitro culture and plant transformation. |
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