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Campylobacter jejuni infection of conventionally colonized mice lacking nucleotide-oligomerization-domain-2

Overview of attention for article published in Gut Pathogens, January 2017
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Title
Campylobacter jejuni infection of conventionally colonized mice lacking nucleotide-oligomerization-domain-2
Published in
Gut Pathogens, January 2017
DOI 10.1186/s13099-017-0155-3
Pubmed ID
Authors

Stefan Bereswill, Ursula Grundmann, Marie E. Alutis, André Fischer, Markus M. Heimesaat

Abstract

The nucleotide-binding oligomerisaton protein 2 (NOD2) constitutes a pivotal sensor of bacterial muramyl dipeptide and assures expression of distinct antimicrobial peptides and mediators produced by enterocytes and immune cells directed against pathogens including Campylobacter jejuni. We here elucidated the role of NOD2 during murine C. jejuni infection in more detail. Conventionally colonized NOD2 deficient (NOD2(-/-)) mice and corresponding wildtype (WT) counterparts were perorally infected with C. jejuni strain 81-176 on three consecutive days. The pathogen colonized both WT and NOD2(-/-) mice only sporadically until day 14 post infection (p.i.). However, the slightly higher prevalence of C. jejuni in NOD2(-/-) mice was accompanied by higher intestinal Escherichia coli loads known to facilitate C. jejuni colonization. Neither overt macroscopic (clinical) nor microscopic sequelae (such as colonic epithelial apoptosis) could be observed upon murine C. jejuni infection of either genotype. Innate immune responses were less distinctly induced in C. jejuni infected NOD2(-/-) versus WT mice as indicated by lower colonic numbers of neutrophils in the former. Conversely, adaptive immune cell counts including T lymphocytes were higher in large intestines of NOD2(-/-) as compared to WT mice that were paralleled by increased colonic IL-6 secretion and higher TNF and IL-18 mRNA expression levels in large intestines of the former. Only in NOD2(-/-) mice, however, colonic IL-22 mRNA expression was down-regulated at day 14 p.i. Whereas viable commensal intestinal bacteria could exclusively be detected in mesenteric lymph nodes and livers of NOD2(-/-) mice, bacterial translocation rates to kidneys and spleen were NOD2 independent. Notably, large intestinal mRNA expression levels of mucin-2, constituting a pivotal factor involved in epithelial barrier integrity, were comparable in naive and C. jejuni infected mice of either genotype. NOD2 is involved in the well-balanced regulation of innate and adaptive pro-inflammatory immune responses of conventional mice upon C. jejuni infection.

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Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 25%
Researcher 4 20%
Student > Ph. D. Student 2 10%
Professor 1 5%
Student > Bachelor 1 5%
Other 1 5%
Unknown 6 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 30%
Medicine and Dentistry 3 15%
Veterinary Science and Veterinary Medicine 1 5%
Immunology and Microbiology 1 5%
Biochemistry, Genetics and Molecular Biology 1 5%
Other 1 5%
Unknown 7 35%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 January 2017.
All research outputs
#7,777,137
of 8,972,546 outputs
Outputs from Gut Pathogens
#216
of 245 outputs
Outputs of similar age
#254,800
of 308,789 outputs
Outputs of similar age from Gut Pathogens
#10
of 13 outputs
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So far Altmetric has tracked 245 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.