Title |
Identification of species belonging to the Bifidobacterium genus by PCR-RFLP analysis of a hsp60 gene fragment
|
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Published in |
BMC Microbiology, July 2013
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DOI | 10.1186/1471-2180-13-149 |
Pubmed ID | |
Authors |
Loredana Baffoni, Verena Stenico, Erwin Strahsburger, Francesca Gaggìa, Diana Di Gioia, Monica Modesto, Paola Mattarelli, Bruno Biavati |
Abstract |
Bifidobacterium represents one of the largest genus within the Actinobacteria, and includes at present 32 species. These species share a high sequence homology of 16S rDNA and several molecular techniques already applied to discriminate among them give ambiguous results.The slightly higher variability of the hsp60 gene sequences with respect to the 16S rRNA sequences offers better opportunities to design or develop molecular assays, allowing identification and differentiation of closely related species. hsp60 can be considered an excellent additional marker for inferring the taxonomy of the members of Bifidobacterium genus. |
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