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Trypsin-induced proteome alteration during cell subculture in mammalian cells

Overview of attention for article published in Journal of Biomedical Science, May 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

Mentioned by

twitter
3 X users
patent
2 patents
wikipedia
5 Wikipedia pages
q&a
1 Q&A thread

Citations

dimensions_citation
235 Dimensions

Readers on

mendeley
587 Mendeley
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Title
Trypsin-induced proteome alteration during cell subculture in mammalian cells
Published in
Journal of Biomedical Science, May 2010
DOI 10.1186/1423-0127-17-36
Pubmed ID
Authors

Hsiang-Ling Huang, Hsiang-Wei Hsing, Tzu-Chia Lai, Yi-Wen Chen, Tian-Ren Lee, Hsin-Tsu Chan, Ping-Chiang Lyu, Chieh-Lin Wu, Ying-Chieh Lu, Szu-Ting Lin, Cheng-Wen Lin, Chih-Ho Lai, Hao-Teng Chang, Hsiu-Chuan Chou, Hong-Lin Chan

Abstract

It is essential to subculture the cells once cultured cells reach confluence. For this, trypsin is frequently applied to dissociate adhesive cells from the substratum. However, due to the proteolytic activity of trypsin, cell surface proteins are often cleaved, which leads to dysregulation of the cell functions.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 587 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 <1%
Kenya 1 <1%
Finland 1 <1%
Portugal 1 <1%
Denmark 1 <1%
United Kingdom 1 <1%
Russia 1 <1%
Croatia 1 <1%
Unknown 578 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 149 25%
Student > Ph. D. Student 116 20%
Student > Master 64 11%
Researcher 52 9%
Student > Postgraduate 19 3%
Other 52 9%
Unknown 135 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 130 22%
Agricultural and Biological Sciences 114 19%
Engineering 46 8%
Medicine and Dentistry 32 5%
Chemistry 32 5%
Other 87 15%
Unknown 146 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 January 2024.
All research outputs
#3,415,054
of 25,373,627 outputs
Outputs from Journal of Biomedical Science
#139
of 1,101 outputs
Outputs of similar age
#12,906
of 103,789 outputs
Outputs of similar age from Journal of Biomedical Science
#1
of 6 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,101 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.0. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 103,789 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them