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Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects

Overview of attention for article published in Genome Biology (Online Edition), February 2017
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#33 of 3,957)
  • High Attention Score compared to outputs of the same age (99th percentile)

Mentioned by

news
28 news outlets
blogs
4 blogs
policy
1 policy source
twitter
31 tweeters
facebook
3 Facebook pages
wikipedia
2 Wikipedia pages
googleplus
1 Google+ user
video
2 video uploaders

Citations

dimensions_citation
116 Dimensions

Readers on

mendeley
236 Mendeley
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Title
Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects
Published in
Genome Biology (Online Edition), February 2017
DOI 10.1186/s13059-016-1144-4
Pubmed ID
Authors

Yuanpeng Gao, Haibo Wu, Yongsheng Wang, Xin Liu, Linlin Chen, Qian Li, Chenchen Cui, Xu Liu, Jingcheng Zhang, Yong Zhang

Abstract

The CRISPR-Cas9 system is a widely utilized platform for transgenic animal production in various species, although its off-target effects should be addressed. Several applications of this tool have been proposed in model animals but remain insufficient for transgenic livestock production. Here, we report the first application of single Cas9 nickase (Cas9n) to induce gene insertion at a selected locus in cattle. We identify the main binding sites of a catalytically inactive Cas9 (dCas9) protein in bovine fetal fibroblast cells (BFFs) with chromatin immunoprecipitation sequencing (ChIP-seq). Subsequently, we demonstrate that a single Cas9n-induced single-strand break can stimulate the insertion of the natural resistance-associated macrophage protein-1 (NRAMP1) gene with reduced, but still considerable, off-target effects. Through somatic cell nuclear transfer, we finally obtain transgenic cattle with increased resistance to tuberculosis. Our results contribute to the development of CRISPR-Cas9 system for agriculture applications.

Twitter Demographics

The data shown below were collected from the profiles of 31 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 236 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Finland 1 <1%
Sweden 1 <1%
France 1 <1%
Unknown 233 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 51 22%
Student > Master 36 15%
Researcher 32 14%
Student > Ph. D. Student 23 10%
Other 10 4%
Other 32 14%
Unknown 52 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 87 37%
Biochemistry, Genetics and Molecular Biology 63 27%
Chemistry 5 2%
Veterinary Science and Veterinary Medicine 4 2%
Social Sciences 4 2%
Other 17 7%
Unknown 56 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 259. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 January 2021.
All research outputs
#100,953
of 21,343,339 outputs
Outputs from Genome Biology (Online Edition)
#33
of 3,957 outputs
Outputs of similar age
#2,939
of 391,594 outputs
Outputs of similar age from Genome Biology (Online Edition)
#1
of 1 outputs
Altmetric has tracked 21,343,339 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,957 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.5. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 391,594 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them