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Physiological and molecular characterizations of the interactions in two cellulose-to-methane cocultures

Overview of attention for article published in Biotechnology for Biofuels, February 2017
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  • Above-average Attention Score compared to outputs of the same age (57th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (58th percentile)

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3 tweeters

Citations

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4 Dimensions

Readers on

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10 Mendeley
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Title
Physiological and molecular characterizations of the interactions in two cellulose-to-methane cocultures
Published in
Biotechnology for Biofuels, February 2017
DOI 10.1186/s13068-017-0719-y
Pubmed ID
Authors

Hongyuan Lu, Siu-Kin Ng, Yangyang Jia, Mingwei Cai, Patrick K. H. Lee

Abstract

The interspecies interactions in a biomethanation community play a vital role in substrate degradation and methane (CH4) formation. However, the physiological and molecular mechanisms of interaction among the microbial members of this community remain poorly understood due to the lack of an experimentally tractable model system. In this study, we successfully established two coculture models combining the cellulose-degrading bacterium Clostridium cellulovorans 743B with Methanosarcina barkeri Fusaro or Methanosarcina mazei Gö1 for the direct conversion of cellulose to CH4. Physiological characterizations of these models revealed that the methanogens in both cocultures were able to efficiently utilize the products produced by C. cellulovorans during cellulose degradation. In particular, the simultaneous utilization of hydrogen, formate, and acetate for methanogenesis was observed in the C. cellulovorans-M. barkeri cocultures, whereas monocultures of M. barkeri were unable to grow with formate alone. Enhanced cellulose degradation was observed in both cocultures, and the CH4 yield of the C. cellulovorans-M. barkeri cocultures (0.87 ± 0.02 mol CH4/mol glucose equivalent) was among the highest compared to other coculture studies. A metabolic shift in the fermentation pattern of C. cellulovorans was observed in both cocultures. The expression levels of genes in key pathways that are important to the regulation and metabolism of the interactions in cocultures were examined by reverse transcription-quantitative PCR, and the expression profiles largely matched the physiological observations. The physiological and molecular characteristics of the interactions of two CH4-producing cocultures are reported. Coculturing C. cellulovorans with M. barkeri or M. mazei not only enabled direct conversion of cellulose to CH4, but also stabilized pH for C. cellulovorans, resulting in a metabolic shift and enhanced cellulose degradation. This study deepens our understanding of interspecies interactions for CH4 production from cellulose, providing useful insights for assembling consortia as inocula for industrial biomethanation processes.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 30%
Student > Doctoral Student 2 20%
Professor 1 10%
Unspecified 1 10%
Student > Master 1 10%
Other 1 10%
Unknown 1 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 20%
Chemical Engineering 1 10%
Environmental Science 1 10%
Unspecified 1 10%
Agricultural and Biological Sciences 1 10%
Other 2 20%
Unknown 2 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 February 2017.
All research outputs
#8,767,468
of 16,206,912 outputs
Outputs from Biotechnology for Biofuels
#532
of 1,191 outputs
Outputs of similar age
#151,427
of 360,375 outputs
Outputs of similar age from Biotechnology for Biofuels
#5
of 12 outputs
Altmetric has tracked 16,206,912 research outputs across all sources so far. This one is in the 45th percentile – i.e., 45% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,191 research outputs from this source. They receive a mean Attention Score of 4.4. This one has gotten more attention than average, scoring higher than 53% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 360,375 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.