Title |
Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli
|
---|---|
Published in |
Microbial Cell Factories, June 2013
|
DOI | 10.1186/1475-2859-12-62 |
Pubmed ID | |
Authors |
Aymerick Eudes, Darmawi Juminaga, Edward E K Baidoo, F William Collins, Jay D Keasling, Dominique Loqué |
Abstract |
Oats contain hydroxycinnamoyl anthranilates, also named avenanthramides (Avn), which have beneficial health properties because of their antioxidant, anti-inflammatory, and antiproliferative effects. The microbial production of hydroxycinnamoyl anthranilates is an eco-friendly alternative to chemical synthesis or purification from plant sources. We recently demonstrated in yeast (Saccharomyces cerevisiae) that coexpression of 4-coumarate: CoA ligase (4CL) from Arabidopsis thaliana and hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase (HCBT) from Dianthus caryophyllusenabled the biological production of several cinnamoyl anthranilates upon feeding with anthranilate and various cinnamates. Using engineering strategies to overproduce anthranilate and hydroxycinnamates, we describe here an entire pathway for the microbial synthesis of two Avns from glucose in Escherichia coli. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Mexico | 1 | 1% |
China | 1 | 1% |
Canada | 1 | 1% |
Unknown | 84 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 25 | 29% |
Student > Ph. D. Student | 20 | 23% |
Student > Master | 7 | 8% |
Student > Bachelor | 6 | 7% |
Professor | 3 | 3% |
Other | 12 | 14% |
Unknown | 14 | 16% |
Readers by discipline | Count | As % |
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Chemistry | 2 | 2% |
Other | 8 | 9% |
Unknown | 17 | 20% |