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Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus

Overview of attention for article published in Virology Journal, February 2017
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Title
Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus
Published in
Virology Journal, February 2017
DOI 10.1186/s12985-017-0688-6
Pubmed ID
Authors

Yang, Xiaodong Qin, Yiming Song, Wei Zhang, Gaowei Hu, Yongxi Dou, Yanmin Li, Zhidong Zhang

Abstract

Peste des petits ruminants (PPR) is an economically important, Office International des Epizooties (OIE) notifiable, transboundary viral disease of small ruminants such as sheep and goat. PPR virus (PPRV), a negative-sense single-stranded RNA virus, is the causal agent of PPR. Therefore, sensitive, specific and rapid diagnostic assay for the detection of PPRV are necessary to accurately and promptly diagnose suspected case of PPR. In this study, reverse transcription recombinase polymerase amplification assays using real-time fluorescent detection (real-time RT-RPA assay) and lateral flow strip detection (LFS RT-RPA assay) were developed targeting the N gene of PPRV. The sensitivity of the developed real-time RT-RPA assay was as low as 100 copies per reaction within 7 min at 40 °C with 95% reliability; while the sensitivity of the developed LFS RT-RPA assay was as low as 150 copies per reaction at 39 °C in less than 25 min. In both assays, there were no cross-reactions with sheep and goat pox viruses, foot-and-mouth disease virus and Orf virus. These features make RPA assay promising candidates either in field use or as a point of care diagnostic technique.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 63 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 21%
Researcher 12 19%
Student > Master 6 10%
Student > Doctoral Student 5 8%
Student > Bachelor 4 6%
Other 9 14%
Unknown 14 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 16%
Veterinary Science and Veterinary Medicine 8 13%
Biochemistry, Genetics and Molecular Biology 6 10%
Chemistry 5 8%
Medicine and Dentistry 4 6%
Other 12 19%
Unknown 18 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 February 2017.
All research outputs
#6,915,148
of 9,075,481 outputs
Outputs from Virology Journal
#1,425
of 1,787 outputs
Outputs of similar age
#186,886
of 253,679 outputs
Outputs of similar age from Virology Journal
#51
of 59 outputs
Altmetric has tracked 9,075,481 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,787 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
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We're also able to compare this research output to 59 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.