Title |
Characterizing the expression of the human olfactory receptor gene family using a novel DNA microarray
|
---|---|
Published in |
Genome Biology, January 2007
|
DOI | 10.1186/gb-2007-8-5-r86 |
Pubmed ID | |
Authors |
Xiaohong Zhang, Omar De la Cruz, Jayant M Pinto, Dan Nicolae, Stuart Firestein, Yoav Gilad |
Abstract |
Olfactory receptor (OR) genes were discovered more than a decade ago, when Buck and Axel observed that, in rats, certain G-protein coupled receptors are expressed exclusively in the olfactory epithelium. Subsequently, protein sequence similarity was used to identify entire OR gene repertoires of a number of mammalian species, but only in mouse were these predictions followed up by expression studies in olfactory epithelium. To rectify this, we have developed a DNA microarray that contains probes for most predicted human OR loci and used that array to examine OR gene expression profiles in olfactory epithelium tissues from three individuals. |
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