Title |
OmpR and RcsB abolish temporal and spatial changes in expression of flhD in Escherichia coli Biofilm
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Published in |
BMC Microbiology, August 2013
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DOI | 10.1186/1471-2180-13-182 |
Pubmed ID | |
Authors |
Priyankar Samanta, Emily R Clark, Katie Knutson, Shelley M Horne, Birgit M Prüß |
Abstract |
Biofilms are communities of bacteria that are characterized by specific phenotypes, including an increased resistance towards anti-microbials and the host immune system. This calls for the development of novel biofilm prevention and treatment options to combat infectious disease. In Escherichia coli, numerous global regulators have been implicated in the control of biofilm associated cell surface organelles. These include the flagellar regulator FlhD/FlhC, the osmoregulator EnvZ/OmpR, and the colanic acid activator RcsCDB. Using flow cell technology and fluorescence microscopy, we determined the temporal expression from flhD::gfp, ompR::gfp, and rcsB::gfp in E. coli biofilm, as well as the impact of the negative regulation of flhD by OmpR and RcsB. Spatial gene expression was investigated from flhD::gfp. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 46 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 24% |
Researcher | 10 | 22% |
Student > Bachelor | 5 | 11% |
Student > Master | 5 | 11% |
Librarian | 1 | 2% |
Other | 2 | 4% |
Unknown | 12 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 14 | 30% |
Biochemistry, Genetics and Molecular Biology | 7 | 15% |
Immunology and Microbiology | 3 | 7% |
Environmental Science | 2 | 4% |
Nursing and Health Professions | 1 | 2% |
Other | 5 | 11% |
Unknown | 14 | 30% |