Title |
The metabolic costs of improving ethanol yield by reducing glycerol formation capacity under anaerobic conditions in Saccharomyces cerevisiae
|
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Published in |
Microbial Cell Factories, March 2013
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DOI | 10.1186/1475-2859-12-29 |
Pubmed ID | |
Authors |
Julien Pagliardini, Georg Hubmann, Sandrine Alfenore, Elke Nevoigt, Carine Bideaux, Stephane E Guillouet |
Abstract |
Finely regulating the carbon flux through the glycerol pathway by regulating the expression of the rate controlling enzyme, glycerol-3-phosphate dehydrogenase (GPDH), has been a promising approach to redirect carbon from glycerol to ethanol and thereby increasing the ethanol yield in ethanol production. Here, strains engineered in the promoter of GPD1 and deleted in GPD2 were used to investigate the possibility of reducing glycerol production of Saccharomyces cerevisiae without jeopardising its ability to cope with process stress during ethanol production. For this purpose, the mutant strains TEFmut7 and TEFmut2 with different GPD1 residual expression were studied in Very High Ethanol Performance (VHEP) fed-batch process under anaerobic conditions. |
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