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An engineered food-grade Lactococcus lactis strain for production and delivery of heat-labile enterotoxin B subunit to mucosal sites

Overview of attention for article published in BMC Biotechnology, March 2017
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Title
An engineered food-grade Lactococcus lactis strain for production and delivery of heat-labile enterotoxin B subunit to mucosal sites
Published in
BMC Biotechnology, March 2017
DOI 10.1186/s12896-017-0345-6
Pubmed ID
Authors

Nan Sun, Rongguang Zhang, Guangcai Duan, Xiaoyan Peng, Chen Wang, Qingtang Fan, Shuaiyin Chen, Yuanlin Xi

Abstract

Recent researches have been focusing on mucosal immune adjuvants, which play the key roles in mucosal immunization and have become the limitation for non-injected vaccine development. Escherichia coli heat-labile enterotoxin B subunit (LTB) was regarded as a promising mucosal adjuvant for its nontoxicity and potent activity. LTB preparation issues have always been recurring, in part owing to that the recombinant LTB expressed by E. coli does not act as its native form. We constructed an engineered Lactococcus lactis strain using a food-grade expression system. The LTB secreted by the engineered strain was detected in the culture supernatant, constituting 10.3% of the supernatant proteins, and recognized by mouse anti-LTB antibodies. The engineered strain, co-administered orally to SPF BALB/c mice with a H. pylori vaccine candidate expressing Lpp20 antigen, could significantly enhance the Lpp20-induced mucosal SIgA antibody responses against H. pylori. This is the first report that LTB was efficiently produced and delivered via using a food-grade lactococcal expression system, which offers a novel production and utilization mode of this crucial mucosal adjuvant. The engineered L. lactis strain secreting LTB has considerable potential for oral vaccine formulation owing to its outstanding safety, adjuvant activity and high-level production.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 21%
Student > Master 3 16%
Student > Postgraduate 3 16%
Student > Bachelor 2 11%
Other 2 11%
Other 1 5%
Unknown 4 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 21%
Medicine and Dentistry 3 16%
Veterinary Science and Veterinary Medicine 2 11%
Immunology and Microbiology 1 5%
Computer Science 1 5%
Other 5 26%
Unknown 3 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2017.
All research outputs
#7,940,478
of 9,159,176 outputs
Outputs from BMC Biotechnology
#456
of 508 outputs
Outputs of similar age
#214,486
of 253,508 outputs
Outputs of similar age from BMC Biotechnology
#7
of 7 outputs
Altmetric has tracked 9,159,176 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 508 research outputs from this source. They receive a mean Attention Score of 4.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one.