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Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain

Overview of attention for article published in Virology Journal, February 2017
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Title
Identification of host cellular proteins that interact with the M protein of a highly pathogenic porcine reproductive and respiratory syndrome virus vaccine strain
Published in
Virology Journal, February 2017
DOI 10.1186/s12985-017-0700-1
Pubmed ID
Authors

Qian Wang, Yanwei Li, Hong Dong, Li Wang, Jinmei Peng, Tongqing An, Xufu Yang, Zhijun Tian, Xuehui Cai

Abstract

The highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) continues to pose one of the greatest threats to the swine industry. M protein is the most conserved and important structural protein of PRRSV. However, information about the host cellular proteins that interact with M protein remains limited. Host cellular proteins that interact with the M protein of HP-PRRSV were immunoprecipitated from MARC-145 cells infected with PRRSV HuN4-F112 using the M monoclonal antibody (mAb). The differentially expressed proteins were identified by LC-MS/MS. The screened proteins were used for bioinformatics analysis including Gene Ontology, the interaction network, and the enriched KEGG pathways. Some interested cellular proteins were validated to interact with M protein by CO-IP. The PRRSV HuN4-F112 infection group had 10 bands compared with the control group. The bands included 219 non-redundant cellular proteins that interact with M protein, which were identified by LC-MS/MS with high confidence. The gene ontology and Kyoto encyclopedia of genes and genomes (KEGG) pathway bioinformatic analyses indicated that the identified proteins could be assigned to several different subcellular locations and functional classes. Functional analysis of the interactome profile highlighted cellular pathways associated with protein translation, infectious disease, and signal transduction. Two interested cellular proteins-nuclear factor of activated T cells 45 kDa (NF45) and proliferating cell nuclear antigen (PCNA)-that could interact with M protein were validated by Co-IP and confocal analyses. The interactome data between PRRSV M protein and cellular proteins were identified and contribute to the understanding of the roles of M protein in the replication and pathogenesis of PRRSV. The interactome of M protein will aid studies of virus/host interactions and provide means to decrease the threat of PRRSV to the swine industry in the future.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 37 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 14%
Student > Master 3 8%
Student > Ph. D. Student 3 8%
Student > Bachelor 2 5%
Professor 1 3%
Other 3 8%
Unknown 20 54%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 6 16%
Agricultural and Biological Sciences 4 11%
Immunology and Microbiology 2 5%
Computer Science 1 3%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Other 3 8%
Unknown 20 54%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 March 2017.
All research outputs
#9,110,152
of 11,379,716 outputs
Outputs from Virology Journal
#1,583
of 1,959 outputs
Outputs of similar age
#188,066
of 257,323 outputs
Outputs of similar age from Virology Journal
#48
of 60 outputs
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So far Altmetric has tracked 1,959 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
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We're also able to compare this research output to 60 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.