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Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses

Overview of attention for article published in Veterinary Research, August 2013
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2 tweeters

Citations

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23 Dimensions

Readers on

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70 Mendeley
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Title
Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline enteric coronaviruses
Published in
Veterinary Research, August 2013
DOI 10.1186/1297-9716-44-71
Pubmed ID
Authors

Lowiese MB Desmarets, Sebastiaan Theuns, Dominique AJ Olyslaegers, Annelike Dedeurwaerder, Ben L Vermeulen, Inge DM Roukaerts, Hans J Nauwynck

Abstract

Feline infectious peritonitis (FIP) is the most feared infectious cause of death in cats, induced by feline infectious peritonitis virus (FIPV). This coronavirus is a virulent mutant of the harmless, ubiquitous feline enteric coronavirus (FECV). To date, feline coronavirus (FCoV) research has been hampered by the lack of susceptible cell lines for the propagation of serotype I FCoVs. In this study, long-term feline intestinal epithelial cell cultures were established from primary ileocytes and colonocytes by simian virus 40 (SV40) T-antigen- and human Telomerase Reverse Transcriptase (hTERT)-induced immortalization. Subsequently, these cultures were evaluated for their usability in FCoV research. Firstly, the replication capacity of the serotype II strains WSU 79-1683 and WSU 79-1146 was studied in the continuous cultures as was done for the primary cultures. In accordance with the results obtained in primary cultures, FCoV WSU 79-1683 still replicated significantly more efficient compared to FCoV WSU 79-1146 in both continuous cultures. In addition, the cultures were inoculated with faecal suspensions from healthy cats and with faecal or tissue suspensions from FIP cats. The cultures were susceptible to infection with different serotype I enteric strains and two of these strains were further propagated. No infection was seen in cultures inoculated with FIPV tissue homogenates. In conclusion, a new reliable model for FCoV investigation and growth of enteric field strains was established. In contrast to FIPV strains, FECVs showed a clear tropism for intestinal epithelial cells, giving an explanation for the observation that FECV is the main pathotype circulating among cats.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Belgium 2 3%
Unknown 68 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 24%
Student > Ph. D. Student 11 16%
Student > Master 11 16%
Student > Bachelor 6 9%
Student > Postgraduate 5 7%
Other 10 14%
Unknown 10 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 24%
Veterinary Science and Veterinary Medicine 12 17%
Immunology and Microbiology 8 11%
Medicine and Dentistry 7 10%
Biochemistry, Genetics and Molecular Biology 6 9%
Other 5 7%
Unknown 15 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 May 2014.
All research outputs
#11,925,380
of 18,796,975 outputs
Outputs from Veterinary Research
#629
of 1,067 outputs
Outputs of similar age
#96,603
of 173,763 outputs
Outputs of similar age from Veterinary Research
#11
of 18 outputs
Altmetric has tracked 18,796,975 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,067 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 173,763 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.