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Histone peptide microarray screen of chromo and Tudor domains defines new histone lysine methylation interactions

Overview of attention for article published in Epigenetics & Chromatin, March 2017
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (77th percentile)

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1 blog
twitter
2 tweeters

Citations

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36 Dimensions

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62 Mendeley
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Title
Histone peptide microarray screen of chromo and Tudor domains defines new histone lysine methylation interactions
Published in
Epigenetics & Chromatin, March 2017
DOI 10.1186/s13072-017-0117-5
Pubmed ID
Authors

Erin K. Shanle, Stephen A. Shinsky, Joseph B. Bridgers, Narkhyun Bae, Cari Sagum, Krzysztof Krajewski, Scott B. Rothbart, Mark T. Bedford, Brian D. Strahl

Abstract

Histone posttranslational modifications (PTMs) function to regulate chromatin structure and function in part through the recruitment of effector proteins that harbor specialized "reader" domains. Despite efforts to elucidate reader domain-PTM interactions, the influence of neighboring PTMs and the target specificity of many reader domains is still unclear. The aim of this study was to use a high-throughput histone peptide microarray platform to interrogate 83 known and putative histone reader domains from the chromo and Tudor domain families to identify their interactions and characterize the influence of neighboring PTMs on these interactions. Nearly a quarter of the chromo and Tudor domains screened showed interactions with histone PTMs by peptide microarray, revealing known and several novel methyllysine interactions. Specifically, we found that the CBX/HP1 chromodomains that recognize H3K9me also recognize H3K23me2/3-a poorly understood histone PTM. We also observed that, in addition to their interaction with H3K4me3, Tudor domains of the Spindlin family also recognized H4K20me3-a previously uncharacterized interaction. Several Tudor domains also showed novel interactions with H3K4me as well. These results provide an important resource for the epigenetics and chromatin community on the interactions of many human chromo and Tudor domains. They also provide the basis for additional studies into the functional significance of the novel interactions that were discovered.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Korea, Republic of 1 2%
Unknown 61 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 26%
Researcher 12 19%
Student > Bachelor 6 10%
Student > Master 4 6%
Student > Doctoral Student 3 5%
Other 9 15%
Unknown 12 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 23 37%
Agricultural and Biological Sciences 11 18%
Chemistry 10 16%
Engineering 2 3%
Medicine and Dentistry 2 3%
Other 3 5%
Unknown 11 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 June 2021.
All research outputs
#3,604,934
of 21,357,544 outputs
Outputs from Epigenetics & Chromatin
#143
of 548 outputs
Outputs of similar age
#63,376
of 277,379 outputs
Outputs of similar age from Epigenetics & Chromatin
#1
of 1 outputs
Altmetric has tracked 21,357,544 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 548 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,379 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them