Title |
Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study
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Published in |
Stem Cell Research & Therapy, March 2017
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DOI | 10.1186/s13287-017-0506-5 |
Pubmed ID | |
Authors |
Misako Nakashima, Koichiro Iohara, Masashi Murakami, Hiroshi Nakamura, Yayoi Sato, Yoshiko Ariji, Kenji Matsushita |
Abstract |
Experiments have previously demonstrated the therapeutic potential of mobilized dental pulp stem cells (MDPSCs) for complete pulp regeneration. The aim of the present pilot clinical study is to assess the safety, potential efficacy, and feasibility of autologous transplantation of MDPSCs in pulpectomized teeth. Five patients with irreversible pulpitis were enrolled and monitored for up to 24 weeks following MDPSC transplantation. The MDPSCs were isolated from discarded teeth and expanded based on good manufacturing practice (GMP). The quality of the MDPSCs at passages 9 or 10 was ascertained by karyotype analyses. The MDPSCs were transplanted with granulocyte colony-stimulating factor (G-CSF) in atelocollagen into pulpectomized teeth. The clinical and laboratory evaluations demonstrated no adverse events or toxicity. The electric pulp test (EPT) of the pulp at 4 weeks demonstrated a robust positive response. The signal intensity of magnetic resonance imaging (MRI) of the regenerated tissue in the root canal after 24 weeks was similar to that of normal dental pulp in the untreated control. Finally, cone beam computed tomography demonstrated functional dentin formation in three of the five patients. Human MDPSCs are safe and efficacious for complete pulp regeneration in humans in this pilot clinical study. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 2 | 20% |
United States | 1 | 10% |
Mexico | 1 | 10% |
Saudi Arabia | 1 | 10% |
Unknown | 5 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 50% |
Scientists | 3 | 30% |
Science communicators (journalists, bloggers, editors) | 1 | 10% |
Practitioners (doctors, other healthcare professionals) | 1 | 10% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 1 | <1% |
Unknown | 337 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 38 | 11% |
Student > Bachelor | 38 | 11% |
Student > Ph. D. Student | 28 | 8% |
Student > Doctoral Student | 22 | 7% |
Student > Postgraduate | 20 | 6% |
Other | 56 | 17% |
Unknown | 136 | 40% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 141 | 42% |
Biochemistry, Genetics and Molecular Biology | 22 | 7% |
Agricultural and Biological Sciences | 8 | 2% |
Materials Science | 4 | 1% |
Neuroscience | 3 | <1% |
Other | 14 | 4% |
Unknown | 146 | 43% |