Title |
Translational HIV-1 research: from routine diagnostics to new virology insights in Amsterdam, the Netherlands during 1983-2013
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Published in |
Retrovirology, August 2013
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DOI | 10.1186/1742-4690-10-93 |
Pubmed ID | |
Authors |
Antoinette C van der Kuyl, Margreet Bakker, Suzanne Jurriaans, Nicole KT Back, Alexander O Pasternak, Marion Cornelissen, Ben Berkhout |
Abstract |
An HIV-1 diagnostic laboratory was established in the Academic Medical Center (AMC) of the University of Amsterdam after the discovery of human immunodeficiency virus (HIV) as the cause of the acquired immunodeficiency syndrome (AIDS). The first AIDS patients were diagnosed here in 1981 and since 1983 we have tested the samples of 50992 patients using a variety of assays that greatly improved over the years. We will describe some of the basic results from this diagnostic laboratory and then focus on the spin-off in terms of the development of novel virus assays to detect super-infections and ultra-sensitive assays to measure the intracellular HIV-1 RNA load. We also review several original research findings in the field of HIV-1 virology that stem from initial observations made in the diagnostic unit. This includes the study of genetic defects in the HIV-1 genome and time trends of the replication fitness over 30 years of viral evolution, but also the description of novel HIV-1 variants in difficult-to-diagnose clinical specimen. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Cameroon | 1 | 4% |
United States | 1 | 4% |
Unknown | 22 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 5 | 21% |
Researcher | 5 | 21% |
Student > Doctoral Student | 2 | 8% |
Student > Postgraduate | 2 | 8% |
Professor | 2 | 8% |
Other | 5 | 21% |
Unknown | 3 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 7 | 29% |
Agricultural and Biological Sciences | 4 | 17% |
Biochemistry, Genetics and Molecular Biology | 2 | 8% |
Social Sciences | 2 | 8% |
Immunology and Microbiology | 2 | 8% |
Other | 4 | 17% |
Unknown | 3 | 13% |