Title |
microclass: an R-package for 16S taxonomy classification
|
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Published in |
BMC Bioinformatics, March 2017
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DOI | 10.1186/s12859-017-1583-2 |
Pubmed ID | |
Authors |
Kristian Hovde Liland, Hilde Vinje, Lars Snipen |
Abstract |
Taxonomic classification based on the 16S rRNA gene sequence is important for the profiling of microbial communities. In addition to giving the best possible accuracy, it is also important to quantify uncertainties in the classifications. We present an R package with tools for making such classifications, where the heavy computations are implemented in C++ but operated through the standard R interface. The user may train classifiers based on specialized data sets, but we also supply a ready-to-use function trained on a comprehensive training data set designed specifically for this purpose. This tool also includes some novel ways to quantify uncertainties in the classifications. Based on input sequences of varying length and quality, we demonstrate how the output from the classifications can be used to obtain high quality taxonomic assignments from 16S sequences within the R computing environment. The package is publicly available at the Comprehensive R Archive Network. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 9 | 17% |
France | 5 | 10% |
Spain | 3 | 6% |
Japan | 3 | 6% |
Chile | 2 | 4% |
Canada | 2 | 4% |
United Kingdom | 2 | 4% |
Belgium | 1 | 2% |
Sweden | 1 | 2% |
Other | 10 | 19% |
Unknown | 14 | 27% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 30 | 58% |
Members of the public | 21 | 40% |
Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 2% |
Denmark | 1 | 2% |
France | 1 | 2% |
Unknown | 60 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 19 | 30% |
Student > Ph. D. Student | 13 | 21% |
Student > Master | 11 | 17% |
Other | 3 | 5% |
Student > Bachelor | 3 | 5% |
Other | 7 | 11% |
Unknown | 7 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 22 | 35% |
Biochemistry, Genetics and Molecular Biology | 15 | 24% |
Computer Science | 7 | 11% |
Immunology and Microbiology | 4 | 6% |
Environmental Science | 2 | 3% |
Other | 5 | 8% |
Unknown | 8 | 13% |