↓ Skip to main content

Replication characteristics of porcine reproductive and respiratory syndrome virus (PRRSV) European subtype 1 (Lelystad) and subtype 3 (Lena) strains in nasal mucosa and cells of the monocytic lineage…

Overview of attention for article published in Veterinary Research, September 2013
Altmetric Badge

About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (52nd percentile)
  • Good Attention Score compared to outputs of the same age and source (75th percentile)

Mentioned by

patent
1 patent

Citations

dimensions_citation
40 Dimensions

Readers on

mendeley
40 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Replication characteristics of porcine reproductive and respiratory syndrome virus (PRRSV) European subtype 1 (Lelystad) and subtype 3 (Lena) strains in nasal mucosa and cells of the monocytic lineage: indications for the use of new receptors of PRRSV (Lena)
Published in
Veterinary Research, September 2013
DOI 10.1186/1297-9716-44-73
Pubmed ID
Authors

Ilias S Frydas, Mieke Verbeeck, Jun Cao, Hans J Nauwynck

Abstract

Recently, it has been demonstrated that subtype 3 strains of European type porcine reproductive and respiratory syndrome virus (PRRSV) are more virulent/pathogenic than subtype 1 strains. This points to differences in the pathogenesis. In the present study, a new polarized nasal mucosa explant system was used to study the invasion of the low virulent subtype 1 PRRSV strain Lelystad (LV) and the highly virulent subtype 3 PRRSV strain Lena at the portal of entry. Different cell types of the monocytic lineage (alveolar macrophages (PAM), cultured blood monocytes and monocyte-derived dendritic cells (moDC)) were enclosed to examine replication kinetics of both strains in their putative target cells. At 0, 12, 24, 48 and 72 hours post inoculation (hpi), virus production was analyzed and the infected cells were quantified and identified. Lena replicated much more efficiently than LV in the nasal mucosa explants and to a lesser extent in PAM. Differences in replication were not found in monocytes and moDC. Confocal microscopy demonstrated that for LV, almost all viral antigen positive cells were CD163⁺Sialoadhesin (Sn)⁺, which were mainly located in the lamina propria of the respiratory mucosa. In Lena-infected nasal mucosa, CD163⁺Sn⁺, CD163⁺Sn⁻ and to a lesser extent CD163⁻Sn⁻ monocytic subtypes were involved in infection. CD163⁺Sn⁻ cells were mostly located within or in the proximity of the epithelium. Our results show that, whereas LV replicates in a restricted subpopulation of CD163⁺Sn⁺ monocytic cells in the upper respiratory tract, Lena hijacks a broader range of subpopulations to spread within the mucosa. Replication in CD163⁺Sn⁻ cells suggests that an alternative entry receptor may contribute to the wider tropism of Lena.

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 2 5%
United States 1 3%
Unknown 37 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 35%
Researcher 5 13%
Student > Master 4 10%
Student > Bachelor 3 8%
Professor 3 8%
Other 7 18%
Unknown 4 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 40%
Veterinary Science and Veterinary Medicine 12 30%
Immunology and Microbiology 4 10%
Biochemistry, Genetics and Molecular Biology 2 5%
Medicine and Dentistry 1 3%
Other 0 0%
Unknown 5 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 April 2018.
All research outputs
#6,927,797
of 21,347,849 outputs
Outputs from Veterinary Research
#349
of 1,139 outputs
Outputs of similar age
#61,285
of 185,527 outputs
Outputs of similar age from Veterinary Research
#3
of 8 outputs
Altmetric has tracked 21,347,849 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,139 research outputs from this source. They receive a mean Attention Score of 4.6. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 185,527 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.