Title |
Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria -assessment of DNA extraction methods and field applicability
|
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Published in |
Malaria Journal, March 2013
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DOI | 10.1186/1475-2875-12-106 |
Pubmed ID | |
Authors |
Ulrika Morris, Berit Aydin-Schmidt, Delér Shakely, Andreas Mårtensson, Louise Jörnhagen, Abdullah S Ali, Mwinyi I Msellem, Max Petzold, José P Gil, Pedro E Ferreira, Anders Björkman |
Abstract |
The need for new malaria surveillance tools and strategies is critical, given improved global malaria control and regional elimination efforts. High quality Plasmodium falciparum DNA can reliably be extracted from malaria rapid diagnostic tests (RDTs). Together with highly sensitive molecular assays, wide scale collection of used RDTs may serve as a modern tool for improved malaria case detection and drug resistance surveillance. However, comparative studies of DNA extraction efficiency from RDTs and the field applicability are lacking. The aim of this study was to compare and evaluate different methods of DNA extraction from RDTs and to test the field applicability for the purpose of molecular epidemiological investigations. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 3 | 3% |
Sudan | 1 | <1% |
Peru | 1 | <1% |
Cambodia | 1 | <1% |
United States | 1 | <1% |
Unknown | 108 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 21 | 18% |
Researcher | 21 | 18% |
Student > Master | 20 | 17% |
Student > Bachelor | 12 | 10% |
Other | 10 | 9% |
Other | 19 | 17% |
Unknown | 12 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 33 | 29% |
Medicine and Dentistry | 25 | 22% |
Biochemistry, Genetics and Molecular Biology | 12 | 10% |
Immunology and Microbiology | 5 | 4% |
Chemistry | 4 | 3% |
Other | 21 | 18% |
Unknown | 15 | 13% |