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Methanol fixation of plant tissue for Scanning Electron Microscopy improves preservation of tissue morphology and dimensions

Overview of attention for article published in Plant Methods, October 2013
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (73rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (55th percentile)

Mentioned by

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4 X users
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1 patent

Citations

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113 Dimensions

Readers on

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238 Mendeley
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Title
Methanol fixation of plant tissue for Scanning Electron Microscopy improves preservation of tissue morphology and dimensions
Published in
Plant Methods, October 2013
DOI 10.1186/1746-4811-9-36
Pubmed ID
Authors

Mark J Talbot, Rosemary G White

Abstract

It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to identify a protocol that would preserve tissue size and morphology better than standard chemical fixatives and dehydration regimes. We compared a range of processing techniques by quantifying changes in tissue size and recording details of surface morphology using leaf tissues from three commonly studied species; Arabidopsis thaliana, barley and cotton.

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 238 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 <1%
Sweden 1 <1%
Brazil 1 <1%
Unknown 235 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 68 29%
Researcher 40 17%
Student > Bachelor 28 12%
Student > Master 27 11%
Professor 8 3%
Other 27 11%
Unknown 40 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 104 44%
Biochemistry, Genetics and Molecular Biology 24 10%
Environmental Science 9 4%
Materials Science 8 3%
Engineering 8 3%
Other 32 13%
Unknown 53 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2018.
All research outputs
#6,930,204
of 25,374,917 outputs
Outputs from Plant Methods
#412
of 1,262 outputs
Outputs of similar age
#58,712
of 220,038 outputs
Outputs of similar age from Plant Methods
#4
of 9 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 1,262 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 220,038 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.