Title |
A sampling framework for incorporating quantitative mass spectrometry data in protein interaction analysis
|
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Published in |
BMC Bioinformatics, October 2013
|
DOI | 10.1186/1471-2105-14-299 |
Pubmed ID | |
Authors |
George Tucker, Po-Ru Loh, Bonnie Berger |
Abstract |
Comprehensive protein-protein interaction (PPI) maps are a powerful resource for uncovering the molecular basis of genetic interactions and providing mechanistic insights. Over the past decade, high-throughput experimental techniques have been developed to generate PPI maps at proteome scale, first using yeast two-hybrid approaches and more recently via affinity purification combined with mass spectrometry (AP-MS). Unfortunately, data from both protocols are prone to both high false positive and false negative rates. To address these issues, many methods have been developed to post-process raw PPI data. However, with few exceptions, these methods only analyze binary experimental data (in which each potential interaction tested is deemed either observed or unobserved), neglecting quantitative information available from AP-MS such as spectral counts. |
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