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Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line

Overview of attention for article published in Cancer Cell International, February 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

blogs
1 blog

Citations

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68 Dimensions

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85 Mendeley
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Title
Cytotoxic and apoptogenic effect of hypericin, the bioactive component of Hypericum perforatum on the MCF-7 human breast cancer cell line
Published in
Cancer Cell International, February 2016
DOI 10.1186/s12935-016-0279-4
Pubmed ID
Authors

Seyed Abbas Mirmalek, Mohammad Amin Azizi, Ehsan Jangholi, Soheila Yadollah-Damavandi, Mohammad Amin Javidi, Yekta Parsa, Tina Parsa, Seyed Alireza Salimi-Tabatabaee, Hossein Ghasemzadeh kolagar, Reza Alizadeh-Navaei

Abstract

Breast cancer is the most prevalent malignancies among the women that have a high mortality. Previous studies demonstrated that hypericin, a bioactive component of Hypericum perforatum have a cytotoxic effect on the malignant cell lines. However, an anti-carcinogenic activity of hypericin on MCF-7 is uncertain. To investigate the cytotoxic effect of hypericin on MCF-7 cells, a human breast adenocarcinoma cell-line, that resistance to chemotherapy. The MCF-7 and fibroblast (as normal cell line) were treated with various concentrations of hypericin, and Cisplatin as a positive control for 24 and 48 h. Cytotoxicity activity was measured and confirmed by MTT assay and Trypan blue staining, respectively. In addition, Apoptosis were determined by Annexin V/Propidium Iodide assay. Immunocytochemistry (ICC) analysis for bcl2 and p53 proteins performed to further investigate different expression of these genes in different samples. Both cisplatin and the hypericin exhibited a dose-dependent cytotoxic effect in the MCF-7 cell line. Although the LD50 of the hypericin was significantly lower when compared to cispaltin (5 vs. 20 μg/ml), it continued to decrease the growth rate of the MCF-7 cells when tested at higher concentration than LD50. In contrast, cisplatine, at higher concentration than LD50, completely inhibited the growth of the MCF-7 in 48 h. Regarding Annexin V/Propidium results, treatment of MCF-7 cells with LD50 concentration of cisplatin and hypericin showed 60 and 52 % apoptosis in 24 h, respectively. ICC analysis for bcl2 and p53 also confirmed our results; in treated samples for the dose of LD50 in 24 and 48 h of cisplatin and hypercin, more cells expressed p53 (guardian of cells in front of tumor formation/progression) and less expressed bcl2 (which has anti apoptotic activity) compared to untreated samples. Considering that hypericin showed to be cytotoxic, it seems to be a chemopreventive agent and a good candidate for antineoplastic drug development.

Mendeley readers

The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 1%
Unknown 84 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 16%
Student > Bachelor 12 14%
Student > Master 10 12%
Professor > Associate Professor 8 9%
Researcher 5 6%
Other 9 11%
Unknown 27 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 17 20%
Agricultural and Biological Sciences 10 12%
Chemistry 9 11%
Pharmacology, Toxicology and Pharmaceutical Science 8 9%
Medicine and Dentistry 4 5%
Other 9 11%
Unknown 28 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2017.
All research outputs
#3,302,145
of 22,961,203 outputs
Outputs from Cancer Cell International
#326
of 1,811 outputs
Outputs of similar age
#63,002
of 401,065 outputs
Outputs of similar age from Cancer Cell International
#1
of 15 outputs
Altmetric has tracked 22,961,203 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,811 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 401,065 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.