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Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus

Overview of attention for article published in Biotechnology for Biofuels, March 2017
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Title
Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus
Published in
Biotechnology for Biofuels, March 2017
DOI 10.1186/s13068-017-0760-x
Pubmed ID
Authors

Yongil Yang, Chang Geun Yoo, Hao-Bo Guo, William Rottmann, Kimberly A. Winkeler, Cassandra M. Collins, Lee E. Gunter, Sara S. Jawdy, Xiaohan Yang, Hong Guo, Yunqiao Pu, Arthur J. Ragauskas, Gerald A. Tuskan, Jin-Gui Chen

Abstract

Domain of Unknown Function 266 (DUF266) is a plant-specific domain. DUF266-containing proteins (DUF266 proteins) have been categorized as 'not classified glycosyltransferases (GTnc)' due to amino acid similarity with GTs. However, little is known about the function of DUF266 proteins. Phylogenetic analysis revealed that DUF266 proteins are only present in the land plants including moss and lycophyte. We report the functional characterization of one member of DUF266 proteins in Populus, PdDUF266A. PdDUF266A was ubiquitously expressed with high abundance in the xylem. In Populus transgenic plants overexpressing PdDUF266A (OXPdDUF266A), the glucose and cellulose contents were significantly higher, while the lignin content was lower than that in the wild type. Degree of polymerization of cellulose in OXPdDUF266A transgenic plants was also higher, whereas cellulose crystallinity index remained unchanged. Gene expression analysis indicated that cellulose biosynthesis-related genes such as CESA and SUSY were upregulated in mature leaf and xylem of OXPdDUF266A transgenic plants. Moreover, PdDUF266A overexpression resulted in an increase of biomass production. Their glucose contents and biomass phenotypes were further validated via heterologous expression of PdDUF266A in Arabidopsis. Results from saccharification treatment demonstrated that the rate of sugar release was increased by approximately 38% in the OXPdDUF266A transgenic plants. These results suggest that the overexpression of PdDUF266A can increase cellulose content, reduce recalcitrance, and enhance biomass production, and that PdDUF266A is a promising target for genetic manipulation for biofuel production.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 24%
Student > Doctoral Student 4 14%
Student > Ph. D. Student 4 14%
Student > Master 3 10%
Professor > Associate Professor 2 7%
Other 3 10%
Unknown 6 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 45%
Biochemistry, Genetics and Molecular Biology 5 17%
Chemistry 2 7%
Chemical Engineering 1 3%
Computer Science 1 3%
Other 2 7%
Unknown 5 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 March 2017.
All research outputs
#6,717,634
of 9,259,051 outputs
Outputs from Biotechnology for Biofuels
#523
of 766 outputs
Outputs of similar age
#172,442
of 259,779 outputs
Outputs of similar age from Biotechnology for Biofuels
#38
of 40 outputs
Altmetric has tracked 9,259,051 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 766 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 259,779 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.