Title |
Poly-L-glutamate/glutamine synthesis in the cell wall of Mycobacterium bovisis regulated in response to nitrogen availability
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Published in |
BMC Microbiology, October 2013
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DOI | 10.1186/1471-2180-13-226 |
Pubmed ID | |
Authors |
Deeksha Tripathi, Harish Chandra, Rakesh Bhatnagar |
Abstract |
The cell wall of pathogenic mycobacteria is known to possess poly-L-glutamine (PLG) layer. PLG synthesis has been directly linked to glutamine synthetase (GS) enzyme. glnA1 gene encodes for GS enzyme in mycobacteria. PLG layer is absent in cell wall of avirulent Mycobacterium smegmatis, although M. smegmatis strain expressing GS enzyme of pathogenic mycobacteria can synthesize PLG layer in the cell wall. The role of GS enzyme has been extensively studied in Mycobacterium tuberculosis, however, little is known about GS enzyme in other mycobacterial species. Mycobacterium bovis, as an intracellular pathogen encounters nitrogen stress inside macrophages, thus it has developed nitrogen assimilatory pathways to survive in adverse conditions. We have investigated the expression and activity of M. bovis GS in response to nitrogen availability and effect on synthesis of PLG layer in the cell wall. M. smegmatis was used as a model to study the behaviour of glnA1 locus of M. bovis. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Spain | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 42 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 21% |
Researcher | 8 | 19% |
Student > Bachelor | 4 | 10% |
Student > Postgraduate | 4 | 10% |
Student > Master | 4 | 10% |
Other | 8 | 19% |
Unknown | 5 | 12% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 16 | 38% |
Biochemistry, Genetics and Molecular Biology | 9 | 21% |
Immunology and Microbiology | 4 | 10% |
Medicine and Dentistry | 2 | 5% |
Nursing and Health Professions | 1 | 2% |
Other | 3 | 7% |
Unknown | 7 | 17% |