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Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000

Overview of attention for article published in Microbial Cell Factories, January 2017
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Title
Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000
Published in
Microbial Cell Factories, January 2017
DOI 10.1186/s12934-016-0616-2
Pubmed ID
Authors

Duolong Zhu, Yuxin Fu, Fulu Liu, Haijin Xu, Per Erik Joakim Saris, Mingqiang Qiao

Abstract

The implementation of novel chassis organisms to be used as microbial cell factories in industrial applications is an intensive research field. Lactococcus lactis, which is one of the most extensively studied model organisms, exhibits superior ability to be used as engineered host for fermentation of desirable products. However, few studies have reported about genome reduction of L. lactis as a clean background for functional genomic studies and a model chassis for desirable product fermentation. Four large nonessential DNA regions accounting for 2.83% in L. lactis NZ9000 (L. lactis 9 k) genome (2,530,294 bp) were deleted using the Cre-loxP deletion system as the first steps toward a minimized genome in this study. The mutants were compared with the parental strain in several physiological traits and evaluated as microbial cell factories for heterologous protein production (intracellular and secretory expression) with the red fluorescent protein (RFP) and the bacteriocin leucocin C (LecC) as reporters. The four mutants grew faster, yielded enhanced biomass, achieved increased adenosine triphosphate content, and diminished maintenance demands compared with the wild strain in the two media tested. In particular, L. lactis 9 k-4 with the largest deletion was identified as the optimum candidate host for recombinant protein production. With nisin induction, not only the transcriptional efficiency but also the production levels of the expressed reporters were approximately three- to fourfold improved compared with the wild strain. The expression of lecC gene controlled with strong constitutive promoters P5 and P8 in L. lactis 9 k-4 was also improved significantly. The genome-streamlined L. lactis 9 k-4 outcompeted the parental strain in several physiological traits assessed. Moreover, L. lactis 9 k-4 exhibited good properties as platform organism for protein production. In future works, the genome of L. lactis will be maximally reduced by using our specific design to provide an even more clean background for functional genomics studies than L. lactis 9 k-4 constructed in this study. Furthermore, an improved background will be potentially available for use in biotechology.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 2%
Denmark 1 2%
Unknown 50 96%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 23%
Student > Ph. D. Student 8 15%
Student > Bachelor 6 12%
Student > Master 6 12%
Other 3 6%
Other 6 12%
Unknown 11 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 18 35%
Agricultural and Biological Sciences 14 27%
Chemical Engineering 2 4%
Philosophy 2 4%
Chemistry 2 4%
Other 3 6%
Unknown 11 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2017.
All research outputs
#8,036,470
of 9,272,034 outputs
Outputs from Microbial Cell Factories
#505
of 660 outputs
Outputs of similar age
#219,564
of 260,733 outputs
Outputs of similar age from Microbial Cell Factories
#11
of 15 outputs
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