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Phenolic compounds isolated from fermented blueberry juice decrease hepatocellular glucose output and enhance muscle glucose uptake in cultured murine and human cells

Overview of attention for article published in BMC Complementary and Alternative Medicine, March 2017
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Title
Phenolic compounds isolated from fermented blueberry juice decrease hepatocellular glucose output and enhance muscle glucose uptake in cultured murine and human cells
Published in
BMC Complementary and Alternative Medicine, March 2017
DOI 10.1186/s12906-017-1650-2
Pubmed ID
Authors

Abir Nachar, Hoda M. Eid, Melinda Vinqvist-Tymchuk, Tri Vuong, Wilhelmina Kalt, Chantal Matar, Pierre S. Haddad

Abstract

We recently reported that blueberry juice fermented (FJ) with Serratia vaccinii bacterium has antidiabetic activities both in vivo and in vitro. The purpose of this project was to elucidate the effect of FJ on glucose homeostasis in liver and skeletal muscle cells and to identify active fractions/compounds responsible for this effect. FJ was fractionated using standard chromatography procedures. Hepatic (H4IIE, HepG2) and skeletal muscle cells (C2C12) were treated with maximum non-toxic concentrations of FJ, fractions and isolated compounds thereof. Glucose-6-phosphatase (G6Pase) activity was measured using glucose oxidase method. To measure glucose uptake and glycogen synthase (GS) activity, radioactive assays were used. Fractionation of FJ yielded seven fractions. FJ and its phenolic fractions F2, F3-1 and F3-2 respectively inhibited G-6Pase by 31, 45, 51 and 26%; activated GS by 2.3-, 2.3-, 2.2- and 2-fold; and stimulated glucose uptake by 19, 25, 18 and 15%, as compared to DMSO vehicle control. Subfractionation of the active fractions yielded 4 compounds (catechol, chlorogenic, gallic and protocatechuic acid). Catechol, yielding the greatest bioactivity in G6Pase and glucose uptake assays, decreased G6Pase activity by 54%, increased GS by 2-fold and stimulated glucose uptake by 44% at 45.5 μM. This study identifies novel potential antidiabetic compounds that can help standardize FJ.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Colombia 1 3%
Unknown 32 97%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 15%
Researcher 4 12%
Student > Postgraduate 3 9%
Student > Master 3 9%
Student > Doctoral Student 2 6%
Other 6 18%
Unknown 10 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 15%
Agricultural and Biological Sciences 4 12%
Medicine and Dentistry 4 12%
Pharmacology, Toxicology and Pharmaceutical Science 2 6%
Nursing and Health Professions 2 6%
Other 5 15%
Unknown 11 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2017.
All research outputs
#7,065,356
of 9,272,034 outputs
Outputs from BMC Complementary and Alternative Medicine
#1,590
of 2,281 outputs
Outputs of similar age
#188,478
of 260,734 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#35
of 49 outputs
Altmetric has tracked 9,272,034 research outputs across all sources so far. This one is in the 13th percentile – i.e., 13% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,281 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.7. This one is in the 17th percentile – i.e., 17% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 260,734 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.