Title |
Development of selectable marker free, insect resistant, transgenic mustard (Brassica juncea) plants using Cre/loxmediated recombination
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Published in |
BMC Biotechnology, October 2013
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DOI | 10.1186/1472-6750-13-88 |
Pubmed ID | |
Authors |
Arpita Bala, Amit Roy, Ayan Das, Dipankar Chakraborti, Sampa Das |
Abstract |
Antibiotic/ herbicide resistant marker genes have been proven to be very useful in plant transformation for the initial selection of desired transgenic events. However, presence of these genes in the genetically modified crops may render the crop less acceptable to the consumers. Among several different approaches, the effectiveness of Cre/lox mediated recombination strategy for selectable marker gene (SMG) elimination has previously been demonstrated by different groups in several plants including Brassica. In the present study exploiting Cre/lox mediated recombination strategy, attempt has been made for selectable marker gene elimination from Allium sativum leaf agglutinin (ASAL) expressing Brassica plants with hemipteran insect resistant phenotype. |
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Unknown | 1 | 100% |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
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Student > Master | 6 | 18% |
Researcher | 4 | 12% |
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Professor | 1 | 3% |
Other | 5 | 15% |
Unknown | 6 | 18% |
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Engineering | 1 | 3% |
Other | 0 | 0% |
Unknown | 6 | 18% |