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Incorporation of a hinge domain improves the expansion of chimeric antigen receptor T cells

Overview of attention for article published in Journal of Hematology & Oncology, March 2017
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (71st percentile)
  • Good Attention Score compared to outputs of the same age and source (68th percentile)

Mentioned by

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16 patents
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3 Wikipedia pages

Citations

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72 Dimensions

Readers on

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152 Mendeley
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2 CiteULike
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Title
Incorporation of a hinge domain improves the expansion of chimeric antigen receptor T cells
Published in
Journal of Hematology & Oncology, March 2017
DOI 10.1186/s13045-017-0437-8
Pubmed ID
Authors

Le Qin, Yunxin Lai, Ruocong Zhao, Xinru Wei, Jianyu Weng, Peilong Lai, Baiheng Li, Simiao Lin, Suna Wang, Qiting Wu, Qiubin Liang, Yangqiu Li, Xuchao Zhang, Yilong Wu, Pentao Liu, Yao Yao, Duanqing Pei, Xin Du, Peng Li

Abstract

Multiple iterations of chimeric antigen receptors (CARs) have been developed, mainly focusing on intracellular signaling modules. However, the effect of non-signaling extracellular modules on the expansion and therapeutic efficacy of CARs remains largely undefined. We generated two versions of CAR vectors, with or without a hinge domain, targeting CD19, mesothelin, PSCA, MUC1, and HER2, respectively. Then, we systematically compared the effect of the hinge domains on the growth kinetics, cytokine production, and cytotoxicity of CAR T cells in vitro and in vivo. During in vitro culture period, the percentages and absolute numbers of T cells expressing the CARs containing a hinge domain continuously increased, mainly through the promotion of CD4+ CAR T cell expansion, regardless of the single-chain variable fragment (scFv). In vitro migration assay showed that the hinges enhanced CAR T cells migratory capacity. The T cells expressing anti-CD19 CARs with or without a hinge had similar antitumor capacities in vivo, whereas the T cells expressing anti-mesothelin CARs containing a hinge domain showed enhanced antitumor activities. Hence, our results demonstrate that a hinge contributes to CAR T cell expansion and is capable of increasing the antitumor efficacy of some specific CAR T cells. Our results suggest potential novel strategies in CAR vector design.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 152 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 152 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 24 16%
Student > Ph. D. Student 23 15%
Researcher 17 11%
Student > Master 14 9%
Student > Doctoral Student 9 6%
Other 17 11%
Unknown 48 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 29 19%
Immunology and Microbiology 20 13%
Medicine and Dentistry 16 11%
Agricultural and Biological Sciences 16 11%
Pharmacology, Toxicology and Pharmaceutical Science 3 2%
Other 17 11%
Unknown 51 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 December 2023.
All research outputs
#4,880,234
of 23,485,204 outputs
Outputs from Journal of Hematology & Oncology
#378
of 1,215 outputs
Outputs of similar age
#85,862
of 309,780 outputs
Outputs of similar age from Journal of Hematology & Oncology
#13
of 41 outputs
Altmetric has tracked 23,485,204 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,215 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.7. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 309,780 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 41 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.