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Deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis Rev 1 vaccine

Overview of attention for article published in Veterinary Research, October 2013
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4 tweeters

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8 Dimensions

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32 Mendeley
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Title
Deletion of the GI-2 integrase and the wbkA flanking transposase improves the stability of Brucella melitensis Rev 1 vaccine
Published in
Veterinary Research, October 2013
DOI 10.1186/1297-9716-44-105
Pubmed ID
Authors

Marcos Mancilla, María-Jesús Grilló, María-Jesús de Miguel, Ignacio López-Goñi, Beatriz San-Román, Ana Zabalza-Baranguá, Ignacio Moriyón

Abstract

Brucella melitensis Rev 1 is the best vaccine available for the prophylaxis of small ruminant brucellosis and, indirectly, for reducing human brucellosis. However, Rev 1 shows anomalously high rates of spontaneous dissociation from smooth (S) to rough (R) bacteria, the latter being inefficacious as vaccines. This S-R instability results from the loss of the O-polysaccharide. To overcome this problem, we investigated whether some recently described mechanisms promoting mutations in O-polysaccharide genes were involved in Rev 1 S-R dissociation. We found that a proportion of Rev 1 R mutants result from genome rearrangements affecting the wbo O-polysaccharide loci of genomic island GI-2 and the wbkA O-polysaccharide glycosyltransferase gene of the wbk region. Accordingly, we mutated the GI-2 int gene and the wbk IS transposase involved in those arrangements, and found that these Rev 1 mutants maintained the S phenotype and showed lower dissociation levels. Combining these two mutations resulted in a strain (Rev 2) displaying a 95% decrease in dissociation with respect to parental Rev 1 under conditions promoting dissociation. Rev 2 did not differ from Rev 1 in the characteristics used in Rev 1 typing (growth rate, colonial size, reactivity with O-polysaccharide antibodies, phage, dye and antibiotic susceptibility). Moreover, Rev 2 and Rev 1 showed similar attenuation and afforded similar protection in the mouse model of brucellosis vaccines. We conclude that mutations targeting genes and DNA sequences involved in spontaneous O-polysaccharide loss enhance the stability of a critical vaccine phenotype and complement the empirical stabilization precautions taken during S Brucella vaccine production.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Chile 1 3%
Spain 1 3%
Norway 1 3%
Unknown 29 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 28%
Student > Ph. D. Student 6 19%
Student > Doctoral Student 3 9%
Librarian 3 9%
Student > Bachelor 2 6%
Other 7 22%
Unknown 2 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 14 44%
Biochemistry, Genetics and Molecular Biology 3 9%
Immunology and Microbiology 3 9%
Medicine and Dentistry 3 9%
Nursing and Health Professions 1 3%
Other 3 9%
Unknown 5 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 November 2013.
All research outputs
#13,339,580
of 21,352,585 outputs
Outputs from Veterinary Research
#625
of 1,138 outputs
Outputs of similar age
#115,226
of 210,360 outputs
Outputs of similar age from Veterinary Research
#31
of 55 outputs
Altmetric has tracked 21,352,585 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,138 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 210,360 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 55 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.