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Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii

Overview of attention for article published in Veterinary Research, April 2017
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3 tweeters

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14 Dimensions

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32 Mendeley
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Title
Permissiveness of bovine epithelial cells from lung, intestine, placenta and udder for infection with Coxiella burnetii
Published in
Veterinary Research, April 2017
DOI 10.1186/s13567-017-0430-9
Pubmed ID
Authors

Katharina Sobotta, Katharina Bonkowski, Elisabeth Liebler-Tenorio, Pierre Germon, Pascal Rainard, Nina Hambruch, Christiane Pfarrer, Ilse D. Jacobsen, Christian Menge

Abstract

Ruminants are the main source of human infections with the obligate intracellular bacterium Coxiella (C.) burnetii. Infected animals shed high numbers of C. burnetii by milk, feces, and birth products. In goats, shedding by the latter route coincides with C. burnetii replication in epithelial (trophoblast) cells of the placenta, which led us to hypothesize that epithelial cells are generally implicated in replication and shedding of C. burnetii. We therefore aimed at analyzing the interactions of C. burnetii with epithelial cells of the bovine host (1) at the entry site (lung epithelium) which govern host immune responses and (2) in epithelial cells of gut, udder and placenta decisive for the quantity of pathogen excretion. Epithelial cell lines [PS (udder), FKD-R 971 (small intestine), BCEC (maternal placenta), F3 (fetal placenta), BEL-26 (lung)] were inoculated with C. burnetii strains Nine Mile I (NMI) and NMII at different cultivation conditions. The cell lines exhibited different permissiveness for C. burnetii. While maintaining cell viability, udder cells allowed the highest replication rates with formation of large cell-filling Coxiella containing vacuoles. Intestinal cells showed an enhanced susceptibility to invasion but supported C. burnetii replication only at intermediate levels. Lung and placental cells also internalized the bacteria but in strikingly smaller numbers. In any of the epithelial cells, both Coxiella strains failed to trigger a substantial IL-1β, IL-6 and TNF-α response. Epithelial cells, with mammary epithelial cells in particular, may therefore serve as a niche for C. burnetii replication in vivo without alerting the host's immune response.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 19%
Researcher 6 19%
Student > Doctoral Student 3 9%
Student > Master 3 9%
Student > Bachelor 2 6%
Other 6 19%
Unknown 6 19%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 12 38%
Agricultural and Biological Sciences 5 16%
Biochemistry, Genetics and Molecular Biology 4 13%
Medicine and Dentistry 2 6%
Unspecified 1 3%
Other 2 6%
Unknown 6 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2017.
All research outputs
#5,605,428
of 10,152,716 outputs
Outputs from Veterinary Research
#391
of 695 outputs
Outputs of similar age
#137,451
of 264,784 outputs
Outputs of similar age from Veterinary Research
#5
of 7 outputs
Altmetric has tracked 10,152,716 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 695 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,784 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.