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Glucocorticoid receptor represses brain-derived neurotrophic factor expression in neuron-like cells

Overview of attention for article published in Molecular Brain, April 2017
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  • Above-average Attention Score compared to outputs of the same age (54th percentile)

Mentioned by

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5 tweeters

Citations

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56 Dimensions

Readers on

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98 Mendeley
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Title
Glucocorticoid receptor represses brain-derived neurotrophic factor expression in neuron-like cells
Published in
Molecular Brain, April 2017
DOI 10.1186/s13041-017-0295-x
Pubmed ID
Authors

Hui Chen, Marc Lombès, Damien Le Menuet

Abstract

Brain-derived neurotrophic factor (BDNF) is involved in many functions such as neuronal growth, survival, synaptic plasticity and memorization. Altered expression levels are associated with many pathological situations such as depression, epilepsy, Alzheimer's, Huntington's and Parkinson's diseases. Glucocorticoid receptor (GR) is also crucial for neuron functions, via binding of glucocorticoid hormones (GCs). GR actions largely overlap those of BDNF. It has been proposed that GR could be a regulator of BDNF expression, however the molecular mechanisms involved have not been clearly defined yet. Herein, we analyzed the effect of a GC agonist dexamethasone (DEX) on BDNF expression in mouse neuronal primary cultures and in the newly characterized, mouse hippocampal BZ cell line established by targeted oncogenesis. Mouse Bdnf gene exhibits a complex genomic structure with 8 untranslated exons (I to VIII) splicing onto one common and unique coding exon IX. We found that DEX significantly downregulated total BDNF mRNA expression by around 30%. Expression of the highly expressed exon IV and VI containing transcripts was also reduced by DEX. The GR antagonist RU486 abolished this effect, which is consistent with specific GR-mediated action. Transient transfection assays allowed us to define a short 275 bp region within exon IV promoter responsible for GR-mediated Bdnf repression. Chromatin immunoprecipitation experiments demonstrated GR recruitment onto this fragment, through unidentified transcription factor tethering. Altogether, GR downregulates Bdnf expression through direct binding to Bdnf regulatory sequences. These findings bring new insights into the crosstalk between GR and BDNF signaling pathways both playing a major role in physiology and pathology of the central nervous system.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 98 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 18 18%
Student > Ph. D. Student 17 17%
Researcher 12 12%
Student > Master 10 10%
Student > Doctoral Student 9 9%
Other 15 15%
Unknown 17 17%
Readers by discipline Count As %
Neuroscience 19 19%
Agricultural and Biological Sciences 13 13%
Medicine and Dentistry 13 13%
Biochemistry, Genetics and Molecular Biology 8 8%
Pharmacology, Toxicology and Pharmaceutical Science 7 7%
Other 13 13%
Unknown 25 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2019.
All research outputs
#8,440,289
of 15,621,847 outputs
Outputs from Molecular Brain
#272
of 776 outputs
Outputs of similar age
#117,819
of 267,752 outputs
Outputs of similar age from Molecular Brain
#1
of 1 outputs
Altmetric has tracked 15,621,847 research outputs across all sources so far. This one is in the 45th percentile – i.e., 45% of other outputs scored the same or lower than it.
So far Altmetric has tracked 776 research outputs from this source. They receive a mean Attention Score of 4.6. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,752 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them