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Development of a microinjection system for RNA interference in the water flea Daphnia pulex

Overview of attention for article published in BMC Biotechnology, November 2013
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • Good Attention Score compared to outputs of the same age and source (73rd percentile)

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1 blog
twitter
1 X user

Citations

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28 Dimensions

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60 Mendeley
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Title
Development of a microinjection system for RNA interference in the water flea Daphnia pulex
Published in
BMC Biotechnology, November 2013
DOI 10.1186/1472-6750-13-96
Pubmed ID
Authors

Chizue Hiruta, Kenji Toyota, Hitoshi Miyakawa, Yukiko Ogino, Shinichi Miyagawa, Norihisa Tatarazako, Joseph R Shaw, Taisen Iguchi

Abstract

The ubiquitous, freshwater microcrustacean Daphnia pulex provides a model system for both human health research and monitoring ecosystem integrity. It is the first crustacean to have a well annotated, reference genome assembly that revealed an unusually high gene count highlighted by a large gene orphanage,-i.e., previously uncharacterized genes. Daphnia are capable of either clonal or sexual reproduction, making them ideally suited for genetic manipulation, but the establishment of gene manipulation techniques is needed to accurately define gene functions. Although previous investigations developed an RNA interference (RNAi) system for one congener D. magna, these methods are not appropriate for D. pulex because of the smaller size of their early embryos. In these studies, we develop RNAi techniques for D. pulex by first determining the optimum culture conditions of their isolated embryos and then applying these conditions to the development of microinjection techniques and proof-of-principle RNAi experiments.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
Mexico 1 2%
United States 1 2%
Unknown 57 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 23%
Student > Bachelor 8 13%
Student > Ph. D. Student 7 12%
Student > Doctoral Student 3 5%
Student > Master 3 5%
Other 7 12%
Unknown 18 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 30%
Biochemistry, Genetics and Molecular Biology 12 20%
Environmental Science 7 12%
Medicine and Dentistry 2 3%
Nursing and Health Professions 1 2%
Other 3 5%
Unknown 17 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 November 2013.
All research outputs
#4,360,858
of 23,881,329 outputs
Outputs from BMC Biotechnology
#212
of 937 outputs
Outputs of similar age
#39,856
of 218,366 outputs
Outputs of similar age from BMC Biotechnology
#7
of 23 outputs
Altmetric has tracked 23,881,329 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 937 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.9. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 218,366 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.