Title |
Development of a microinjection system for RNA interference in the water flea Daphnia pulex
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Published in |
BMC Biotechnology, November 2013
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DOI | 10.1186/1472-6750-13-96 |
Pubmed ID | |
Authors |
Chizue Hiruta, Kenji Toyota, Hitoshi Miyakawa, Yukiko Ogino, Shinichi Miyagawa, Norihisa Tatarazako, Joseph R Shaw, Taisen Iguchi |
Abstract |
The ubiquitous, freshwater microcrustacean Daphnia pulex provides a model system for both human health research and monitoring ecosystem integrity. It is the first crustacean to have a well annotated, reference genome assembly that revealed an unusually high gene count highlighted by a large gene orphanage,-i.e., previously uncharacterized genes. Daphnia are capable of either clonal or sexual reproduction, making them ideally suited for genetic manipulation, but the establishment of gene manipulation techniques is needed to accurately define gene functions. Although previous investigations developed an RNA interference (RNAi) system for one congener D. magna, these methods are not appropriate for D. pulex because of the smaller size of their early embryos. In these studies, we develop RNAi techniques for D. pulex by first determining the optimum culture conditions of their isolated embryos and then applying these conditions to the development of microinjection techniques and proof-of-principle RNAi experiments. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Japan | 1 | 2% |
Mexico | 1 | 2% |
United States | 1 | 2% |
Unknown | 57 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 14 | 23% |
Student > Bachelor | 8 | 13% |
Student > Ph. D. Student | 7 | 12% |
Student > Doctoral Student | 3 | 5% |
Student > Master | 3 | 5% |
Other | 7 | 12% |
Unknown | 18 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 18 | 30% |
Biochemistry, Genetics and Molecular Biology | 12 | 20% |
Environmental Science | 7 | 12% |
Medicine and Dentistry | 2 | 3% |
Nursing and Health Professions | 1 | 2% |
Other | 3 | 5% |
Unknown | 17 | 28% |