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A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings

Overview of attention for article published in Plant Methods, April 2017
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  • Above-average Attention Score compared to outputs of the same age (64th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

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7 X users

Citations

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90 Dimensions

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191 Mendeley
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Title
A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings
Published in
Plant Methods, April 2017
DOI 10.1186/s13007-017-0174-6
Pubmed ID
Authors

Ying Liang, Daisuke Urano, Kang-Ling Liao, Tyson L. Hedrick, Yajun Gao, Alan M. Jones

Abstract

Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotypes of mutations and treatments. However, chlorophyll measurements with conventional solvent extraction are not applicable to Arabidopsis leaves due to their small size, especially when grown on culture dishes. We provide a nondestructive method for chlorophyll measurement whereby the red, green and blue (RGB) values of a color leaf image is used to estimate the chlorophyll content from Arabidopsis leaves. The method accommodates different profiles of digital cameras by incorporating the ColorChecker chart to make the digital negative profiles, to adjust the white balance, and to calibrate the exposure rate differences caused by the environment so that this method is applicable in any environment. We chose an exponential function model to estimate chlorophyll content from the RGB values, and fitted the model parameters with physical measurements of chlorophyll contents. As proof of utility, this method was used to estimate chlorophyll content of G protein mutants grown on different sugar to nitrogen ratios. This method is a simple, fast, inexpensive, and nondestructive estimation of chlorophyll content of Arabidopsis seedlings. This method lead to the discovery that G proteins are important in sensing the C/N balance to control chlorophyll content in Arabidopsis.

X Demographics

X Demographics

The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 191 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 191 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 43 23%
Researcher 23 12%
Student > Master 20 10%
Student > Bachelor 19 10%
Student > Doctoral Student 8 4%
Other 23 12%
Unknown 55 29%
Readers by discipline Count As %
Agricultural and Biological Sciences 55 29%
Biochemistry, Genetics and Molecular Biology 32 17%
Engineering 12 6%
Environmental Science 4 2%
Chemistry 3 2%
Other 22 12%
Unknown 63 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2021.
All research outputs
#7,261,399
of 25,163,238 outputs
Outputs from Plant Methods
#457
of 1,237 outputs
Outputs of similar age
#107,146
of 314,680 outputs
Outputs of similar age from Plant Methods
#13
of 34 outputs
Altmetric has tracked 25,163,238 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 1,237 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has gotten more attention than average, scoring higher than 62% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 314,680 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.